| http://purl.uniprot.org/citations/10029690 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10029690 | http://www.w3.org/2000/01/rdf-schema#comment | "Tk+/-transgenic mice were created using an embryonic stem cell line in which one allele of the endogenous thymidine kinase (Tk) gene was inactivated by targeted homologous recombination. Breeding Tk+/- parents produced viable Tk-/-knockout (KO) mice. Splenic lymphocytes from KO mice were used in reconstruction experiments for determining the conditions necessary for recovering Tk somatic cell mutants from Tk+/-mice. The cloning efficiency of KO lymphocytes was not affected by the toxic thymidine analogues 5-bromo-2'-deoxyuridine (BrdUrd) or trifluorothymidine (TFT), or by BrdUrd in the presence of lymphocytes from Tk+/-animals; however, it was easier to identify clones resistant to BrdUrd than to TFT when Tk+/- cells were present. Tk+/-mice were treated with vehicle or 100 mg/kg of N-ethyl-N-nitrosourea (ENU), and after 4 months, the frequency of Tk mutant lymphocytes was measured by resistance to BrdUrd. The frequency of Tk mutants was 22+/-5.9x10-6 in control animals and 80+/-31x10-6 in treated mice. In comparison, the frequency of Hprt mutant lymphocytes, as measured by resistance to 6-thioguanine, was 2.0+/-1.2x10-6 in control animals and 84+/-28x10-6 in the ENU-treated mice. Analysis of BrdUrd-resistant lymphocyte clones derived from the ENU-treated animals revealed point mutations in the non-targeted Tk allele. These results indicate that the selection of BrdUrd-resistant lymphocytes from Tk+/-mice may be used for assessing in vivo mutation in an endogenous, autosomal gene."xsd:string |
| http://purl.uniprot.org/citations/10029690 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0027-5107(98)00234-6"xsd:string |
| http://purl.uniprot.org/citations/10029690 | http://purl.uniprot.org/core/author | "Heflich R.H."xsd:string |
| http://purl.uniprot.org/citations/10029690 | http://purl.uniprot.org/core/author | "Dobrovolsky V.N."xsd:string |
| http://purl.uniprot.org/citations/10029690 | http://purl.uniprot.org/core/author | "Casciano D.A."xsd:string |
| http://purl.uniprot.org/citations/10029690 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10029690 | http://purl.uniprot.org/core/name | "Mutat Res"xsd:string |
| http://purl.uniprot.org/citations/10029690 | http://purl.uniprot.org/core/pages | "125-136"xsd:string |
| http://purl.uniprot.org/citations/10029690 | http://purl.uniprot.org/core/title | "Tk+/- mouse model for detecting in vivo mutation in an endogenous, autosomal gene."xsd:string |
| http://purl.uniprot.org/citations/10029690 | http://purl.uniprot.org/core/volume | "423"xsd:string |
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