| http://purl.uniprot.org/citations/10036242 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10036242 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10036242 | http://www.w3.org/2000/01/rdf-schema#comment | "The replication checkpoint (or 'S-M checkpoint') control prevents progression into mitosis when DNA replication is incomplete. Caffeine has been known for some time to have the capacity to override the S-M checkpoint in animal cells. We show here that caffeine also disrupts the S-M checkpoint in the fission yeast Schizosaccharomyces pombe. By contrast, no comparable effects of caffeine on the S. pombe DNA damage checkpoint were seen. S. pombe cells arrested in early S phase and then exposed to caffeine lost viability rapidly as they attempted to enter mitosis, which was accompanied by tyrosine dephosphorylation of Cdc2. Despite this, the caffeine-induced loss of viability was not blocked in a temperature-sensitive cdc2 mutant incubated at the restrictive temperature, although catastrophic mitosis was prevented under these conditions. This suggests that, in addition to S-M checkpoint control, a caffeine-sensitive function may be important for maintenance of cell viability during S phase arrest. The lethality of a combination of caffeine with the DNA replication inhibitor hydroxyurea was suppressed by overexpression of Cds1 or Chk1, protein kinases previously implicated in S-M checkpoint control and recovery from S phase arrest. In addition, the same combination of drugs was specifically tolerated in cells overexpressing either of two novel S. pombe genes isolated in a cDNA library screen. These findings should allow further molecular investigation of the regulation of S phase arrest, and may provide a useful system with which to identify novel drugs that specifically abrogate the checkpoint control."xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.112.6.927"xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.112.6.927"xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/author | "Toda T."xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/author | "Toda T."xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/author | "Harris A.L."xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/author | "Harris A.L."xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/author | "Norbury C."xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/author | "Norbury C."xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/author | "Wang S.-W."xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/author | "Wang S.-W."xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/pages | "927-937"xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/pages | "927-937"xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/title | "Caffeine can override the S-M checkpoint in fission yeast."xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/title | "Caffeine can override the S-M checkpoint in fission yeast."xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/volume | "112"xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://purl.uniprot.org/core/volume | "112"xsd:string |
| http://purl.uniprot.org/citations/10036242 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10036242 |
| http://purl.uniprot.org/citations/10036242 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10036242 |