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http://purl.uniprot.org/citations/10048395http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10048395http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10048395http://www.w3.org/2000/01/rdf-schema#comment"We are developing a method of gene therapy of HIV infection based on the low constitutive expression of an interferon beta (IFN-beta) gene in HIV target cells. Herein we report the first step in the development of a relevant animal model, provided by the macaque (Macaca fascicularis) infected with a pathogenic SIVmac251 isolate. To avoid the possibility of in vivo rejection of macaque lymphocytes expressing Hu IFN-beta, we have PCR-amplified and sequenced the Ma IFN-beta-coding sequence, and placed it under the control of a PstI-NruI 0.6-kb fragment of the murine H-2Kb gene promoter in the MFG-K(b)MaIFNbeta retroviral vector. Lymphocytic CEMX174 cells, transduced by coculture on packaging cells with this construct, harbored a mean of 0.07 to 1.2 copies of the IFN-beta transgene per cell, and were characterized by an IFN production ranging from 75 to 750 units per 5 x 10(5) cells per 3 days. The IFN-beta-transduced populations displayed an enhanced resistance against the pathogenic SIVmac251 isolate. Control experiments showed that the enhanced resistance could not be ascribed to the Ma IFN-beta released during the 3 days of coculture by the packaging cells, or to the mere transduction with a retroviral vector. Macaque lymphocytes transduced by the MFG-K(b)MaIFNbeta retroviral vector by coculture on packaging cells, acquired a mean number of IFN-beta transgene copies per cell ranging from 0.03 to 0.1. Such transduction led to the release of IFN-beta into the culture medium, ranging from 10 to 20 units per 5 x 10(5) cells per 3 days. This increased the anti-SIV resistance of the lymphocytes, as demonstrated by a decreased p27 antigen release into the culture medium, without affecting lymphocyte proliferation."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.org/dc/terms/identifier"doi:10.1089/10430349950018878"xsd:string
http://purl.uniprot.org/citations/10048395http://purl.org/dc/terms/identifier"doi:10.1089/10430349950018878"xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/author"Dormont D."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/author"Dormont D."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/author"Le Grand R."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/author"Le Grand R."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/author"Lauret E."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/author"Lauret E."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/author"Rousseau V."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/author"Rousseau V."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/author"De Maeyer E."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/author"De Maeyer E."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/author"Matheux F."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/author"Matheux F."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/name"Hum. Gene Ther."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/name"Hum. Gene Ther."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/pages"429-440"xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/pages"429-440"xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/title"Macaque lymphocytes transduced by a constitutively expressed interferon beta gene display an enhanced resistance to SIVmac251 infection."xsd:string
http://purl.uniprot.org/citations/10048395http://purl.uniprot.org/core/title"Macaque lymphocytes transduced by a constitutively expressed interferon beta gene display an enhanced resistance to SIVmac251 infection."xsd:string
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