| http://purl.uniprot.org/citations/10051443 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10051443 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10051443 | http://www.w3.org/2000/01/rdf-schema#comment | "SNARE proteins are required for vesicle docking and fusion in eukaryotic cells in processes as diverse as homotypic membrane fusion and synaptic vesicle exocytosis [SNARE stands for SNAP receptor, where SNAP is soluble NSF attachment protein]. The SNARE proteins syntaxin 4 and vesicle-associated membrane protein (VAMP) 2/3 also participate in the insulin-stimulated translocation of GLUT4 from intracellular vesicles to the plasma membrane in adipose cells. We now report the molecular cloning and characterization of rat SNAP-23, a ubiquitously expressed homologue of the essential neuronal SNARE protein SNAP-25 (synaptosomal-associated protein of 25 kDa). Rat SNAP-23 is 86% and 98% identical respectively to human and mouse SNAP-23. Southern blot analysis reveals that the rat, mouse and human SNAP-23 genes encode species-specific isoforms of the same protein. Co-immunoprecipitation of syntaxin 4 and SNAP-23 shows association of these two proteins in rat adipose cell plasma membranes, and insulin stimulation does not alter the SNAP-23/syntaxin 4 complex. In addition, we demonstrate for the first time the participation of SNAP-23, along with syntaxin 4 and VAMP2/3, in the formation of 20S SNARE complexes prepared using rat adipose cell membranes and recombinant alpha-SNAP and NSF proteins. The stoichiometry of the SNARE complexes formed is essentially identical using membranes from either unstimulated or insulin-stimulated adipose cells. These data demonstrate that rat SNAP-23 associates with syntaxin 4 before insulin stimulation and is present in the SNARE complexes known to mediate the translocation of GLUT4 from intracellular vesicles to the plasma membrane of rat adipose cells."xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3380709"xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3380709"xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/author | "Roche P.A."xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/author | "Roche P.A."xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/author | "Cushman S.W."xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/author | "Cushman S.W."xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/author | "Cabaniols J.-P."xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/author | "Cabaniols J.-P."xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/author | "St Denis J.-F."xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/author | "St Denis J.-F."xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/pages | "709-715"xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/pages | "709-715"xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/title | "SNAP-23 participates in SNARE complex assembly in rat adipose cells."xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/title | "SNAP-23 participates in SNARE complex assembly in rat adipose cells."xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/volume | "338"xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://purl.uniprot.org/core/volume | "338"xsd:string |
| http://purl.uniprot.org/citations/10051443 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10051443 |
| http://purl.uniprot.org/citations/10051443 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10051443 |