| http://purl.uniprot.org/citations/10085297 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10085297 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10085297 | http://www.w3.org/2000/01/rdf-schema#comment | "We recently isolated a novel actin filament (F-actin)-binding protein, afadin, that has two isoforms, l- and s-afadins. l-Afadin is ubiquitously expressed and specifically localized at zonula adherens (ZA) in epithelial cells and at cell-cell adherens junction (AJ) in nonepithelial cells, whereas s-afadin is abundantly expressed in neural tissue. l-Afadin has one PDZ domain, three proline-rich regions, and one F-actin-binding domain, whereas s-afadin lacks the third proline-rich region and the F-actin-binding domain. To understand the molecular mechanism of the specific localization of l-afadin at ZA in epithelial cells and at cell-cell AJ in nonepithelial cells, we attempted here to identify an l-afadin-binding protein(s) and isolated a protein, named ponsin. Ponsin had many splicing variants and the primary structures of two of them were determined. Both the two variants had three Src homology 3 (SH3) domains and turned out to be splicing variants of SH3P12. The third proline-rich region of l-afadin bound to the region of ponsin containing the second and third SH3 domains. Ponsin was ubiquitously expressed and localized at ZA in epithelial cells, at cell-cell AJ in nonepithelial cells, and at cell-matrix AJ in both types of cells. Ponsin furthermore directly bound vinculin, an F-actin-binding protein localized at ZA in epithelial cells, at cell-cell AJ in nonepithelial cells, and at cell-matrix AJ in both types of cells. Vinculin has one proline-rich region where two proline-rich sequences are located. The proline-rich region bound to the region of ponsin containing the first and second SH3 domains. l-Afadin and vinculin bound to ponsin in a competitive manner and these three proteins hardly formed a ternary complex. These results indicate that ponsin is an l-afadin- and vinculin-binding protein localized at ZA in epithelial cells, at cell-cell AJ in nonepithelial cells, and at cell-matrix AJ in both types of cells."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.144.5.1001"xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.144.5.1001"xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Satoh K."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Satoh K."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Takai Y."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Takai Y."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Takahashi K."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Takahashi K."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Nakanishi H."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Nakanishi H."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Nishioka H."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Nishioka H."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Satoh A."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Satoh A."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Mandai K."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Mandai K."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Mizoguchi A."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/author | "Mizoguchi A."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/10085297 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |