| http://purl.uniprot.org/citations/10089391 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10089391 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10089391 | http://www.w3.org/2000/01/rdf-schema#comment | "The crystal structure of a bifunctional inhibitor of alpha-amylase and trypsin from the seeds of ragi (Indian finger millet, Eleusine coracana Gaertneri) has been determined by an X-ray diffraction method. The inhibitor consists of 122 amino acids with five disulfide bridges and belongs to the plant alpha-amylase/trypsin-inhibitor family. This is the first crystal structure determination of a member of this family. The protein, purified from the seeds of ragi, has a molecular mass of 13300 Da with a pI of 10.3. Crystals were grown by a microdialysis method using ammonium sulfate as precipitant. The improved purification protocol and the modified crystallization conditions enabled reproducible growth of the crystals. The cell parameters are a = 41. 2, b = 47.4, c = 55.9 A. The intensity data were collected to 2.9 A resolution, and the crystal structure was determined using the molecular-replacement method. The structure was refined using the X-PLOR and CCP4 program packages to a conventional R factor of 21%. The structure contains four alpha-helices between residues 19-29, 37-51, 56-65 and 90-95, and two short antiparallel beta-strands between residues 67-70 and 73-75."xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.org/dc/terms/identifier | "doi:10.1107/s0907444998006271"xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.org/dc/terms/identifier | "doi:10.1107/s0907444998006271"xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/author | "Singh T.P."xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/author | "Singh T.P."xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/author | "Srinivasan A."xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/author | "Srinivasan A."xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/author | "Gourinath S."xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/author | "Gourinath S."xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/name | "Acta Crystallogr. D"xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/name | "Acta Crystallogr. D"xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/pages | "25-30"xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/pages | "25-30"xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/title | "Structure of the bifunctional inhibitor of trypsin and alpha-amylase from ragi seeds at 2.9-A resolution."xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/title | "Structure of the bifunctional inhibitor of trypsin and alpha-amylase from ragi seeds at 2.9-A resolution."xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/volume | "55"xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://purl.uniprot.org/core/volume | "55"xsd:string |
| http://purl.uniprot.org/citations/10089391 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10089391 |
| http://purl.uniprot.org/citations/10089391 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10089391 |
| http://purl.uniprot.org/citations/10089391 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10089391 |
| http://purl.uniprot.org/citations/10089391 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10089391 |