| http://purl.uniprot.org/citations/10095107 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10095107 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10095107 | http://www.w3.org/2000/01/rdf-schema#comment | "6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK-2/FBPase-2) is a bifunctional enzyme responsible for the synthesis and breakdown of Fru-2,6-P2, a key metabolite in the regulation of glycolysis. Several genes encode distinct PFK-2/FBPase-2 isozymes that differ in their tissue distribution and enzyme regulation. In this paper, we present the isolation of a cDNA from a human testis cDNA library that encodes a PFK-2/FBPase-2 isozyme. Sequencing data show an open reading frame of 1407 nucleotides that codifies for a protein of 469 amino acids. This has a calculated molecular weight of 54kDa and 97% similarity with rat testis PFK-2/FBPase-2, with complete conservation of the amino acid residues involved in the catalytic mechanism. Fluorescence in-situ hybridization (FISH) localized testis PFK-2/FBPase-2 gene (PFKFB4) in human chromosome 3 at bands p21-p22. A Northern blot analysis of different rat tissues showed the presence of a 2.4-kb mRNA expressed specifically in testis. In mammalian COS-1 cells, the human testis cDNA drives expression of an isozyme with a molecular weight of 55kDa. This isozyme shows clear PFK-2 activity. Taken together, these results provide evidence for a new PFK-2/FBPase-2 gene coding for a human testis isozyme."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0378-1119(99)00037-2"xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0378-1119(99)00037-2"xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/author | "Nadal M."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/author | "Nadal M."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/author | "Estivill X."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/author | "Estivill X."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/author | "Bartrons R."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/author | "Bartrons R."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/author | "Manzano A."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/author | "Manzano A."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/author | "Lange A."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/author | "Lange A."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/author | "Perez J.X."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/author | "Perez J.X."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/pages | "83-89"xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/pages | "83-89"xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/title | "Cloning, expression and chromosomal localization of a human testis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene."xsd:string |
| http://purl.uniprot.org/citations/10095107 | http://purl.uniprot.org/core/title | "Cloning, expression and chromosomal localization of a human testis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene."xsd:string |