| http://purl.uniprot.org/citations/10098851 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10098851 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10098851 | http://www.w3.org/2000/01/rdf-schema#comment | "GH3 cells present spontaneous Ca2+ action potentials and oscillations of intracellular Ca2+, which can be modified by altering the activity of K+ or Ca2+ channels. We took advantage of this spontaneous activity to screen for effects of a purified toxin (Tx3-1) from the venom of Phoneutria nigriventer on ion channels. We report that Tx3-1 increases the frequency of Ca2+ oscillations, as do two blockers of potassium channels, 4-aminopyridine and charybdotoxin. Whole-cell patch clamp experiments show that Tx3-1 reversibly inhibits the A-type K+ current (I(A)) but does not block other K+ currents (delayed-rectifying, inward-rectifying, and large-conductance Ca2+-sensitive) or Ca2+ channels (T and L type) in these cells. In addition, we describe the sequence of a full cDNA clone of Tx3-1, which shows that Tx3-1 has no homology to other known blockers of K+ channels and gives insights into the processing of this neurotoxin. We conclude that Tx3-1 is a selective inhibitor of I(A), which can be used to probe the role of this channel in the control of cellular function. Based on the effect of Tx3-1, we suggest that I(A) is an important determinant of the frequency of Ca2+ oscillations in unstimulated GH3 cells."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1471-4159.1999.721472.x"xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1471-4159.1999.721472.x"xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Cordeiro M.N."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Cordeiro M.N."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Kushmerick C."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Kushmerick C."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Gomez M.V."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Gomez M.V."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Kalapothakis E."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Kalapothakis E."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Beirao P.S.L."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Beirao P.S.L."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Cruz J.S."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Cruz J.S."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Prado V.F."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Prado V.F."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Diniz C.R."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Diniz C.R."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Prado M.A.M."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Prado M.A.M."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Romano-Silva M.A."xsd:string |
| http://purl.uniprot.org/citations/10098851 | http://purl.uniprot.org/core/author | "Romano-Silva M.A."xsd:string |