| http://purl.uniprot.org/citations/10187838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10187838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10187838 | http://www.w3.org/2000/01/rdf-schema#comment | "A partial-length human cDNA with a predicted amino acid sequence homologous to a previously described heparan sulfate iduronyl 2-sulfotransferase (Kobayashi, M., Habuchi, H., Yoneda, M., Habuchi, O., and Kimata, K. (1997) J. Biol. Chem. 272, 13980-13985) was obtained by searching the expressed sequence-tagged data bank. Northern blot analysis was performed using this homologous cDNA as a probe, which demonstrated ubiquitous expression of messages of 5.1 and 2.0 kilobases in a number of human tissues and in several human cancer cell lines. Since the human lymphoma Raji cell line had the highest level of expression, it was used to isolate a full-length cDNA clone. The full-length cDNA was found to contain an open reading frame that predicted a type II transmembrane protein composed of 406 amino acid residues. The cDNA in a baculovirus expression vector was expressed in Sf9 insect cells, and cell extracts were then incubated together with 3'-phosphoadenosine 5'-phospho[35S]sulfate and potential glycosaminoglycan acceptors. This demonstrated substantial sulfotransferase activity with dermatan sulfate, a small degree of activity with chondroitin sulfate, but no sulfotransferase activity with desulfated N-resulfated heparin. Analysis of [35S]sulfate-labeled disaccharide products of chondroitin ABC, chondroitin AC, and chondroitin B lyase treatment demonstrated that the enzyme only transferred sulfate to the 2-position of uronyl residues, which were preponderantly iduronyl residues in dermatan sulfate, but some lesser transfer to glucuronyl residues of chondroitin sulfate."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.15.10474"xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.15.10474"xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/author | "Kobayashi M."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/author | "Kobayashi M."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/author | "Liu J."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/author | "Liu J."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/author | "Rosenberg R.D."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/author | "Rosenberg R.D."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/author | "Shworak N.W."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/author | "Shworak N.W."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/author | "Silbert J.E."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/author | "Silbert J.E."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/author | "Sugumaran G."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/author | "Sugumaran G."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/pages | "10474-10480"xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/pages | "10474-10480"xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/title | "Molecular cloning and characterization of a human uronyl 2-sulfotransferase that sulfates iduronyl and glucuronyl residues in dermatan/chondroitin sulfate."xsd:string |
| http://purl.uniprot.org/citations/10187838 | http://purl.uniprot.org/core/title | "Molecular cloning and characterization of a human uronyl 2-sulfotransferase that sulfates iduronyl and glucuronyl residues in dermatan/chondroitin sulfate."xsd:string |