| http://purl.uniprot.org/citations/10189376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10189376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10189376 | http://www.w3.org/2000/01/rdf-schema#comment | "Profilin is an ubiquitous G-actin binding protein in eukaryotic cells. Lack of both profilin isoforms in Dictyostelium discoideum resulted in impaired cytokinesis and an arrest in development. A restriction enzyme-mediated integration approach was applied to profilin-minus cells to identify suppressor mutants for the developmental phenotype. A mutant with wild-type-like development and restored cytokinesis was isolated. The gene affected was found to code for an integral membrane glycoprotein of a predicted size of 88 kD containing two transmembrane domains, one at the NH2 terminus and the other at the COOH terminus. It is homologous to mammalian CD36/LIMP-II and represents the first member of this family in D. discoideum, therefore the name DdLIMP is proposed. Targeted disruption of the lmpA gene in the profilin-minus background also rescued the mutant phenotype. Immunofluorescence revealed a localization in vesicles and ringlike structures on the cell surface. Partially purified DdLIMP bound specifically to PIP2 in sedimentation and gel filtration assays. A direct interaction between DdLIMP and profilin could not be detected, and it is unclear how far upstream in a regulatory cascade DdLIMP might be positioned. However, the PIP2 binding of DdLIMP points towards a function via the phosphatidylinositol pathway, a major regulator of profilin."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.145.1.167"xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.145.1.167"xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/author | "Eichinger L."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/author | "Eichinger L."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/author | "Janssen K.-P."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/author | "Janssen K.-P."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/author | "Noegel A.A."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/author | "Noegel A.A."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/author | "Schleicher M."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/author | "Schleicher M."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/author | "Karakesisoglou I."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/author | "Karakesisoglou I."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/pages | "167-181"xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/pages | "167-181"xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/title | "Identification of a suppressor of the Dictyostelium profilin-minus phenotype as a CD36/LIMP-II homologue."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/title | "Identification of a suppressor of the Dictyostelium profilin-minus phenotype as a CD36/LIMP-II homologue."xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/volume | "145"xsd:string |
| http://purl.uniprot.org/citations/10189376 | http://purl.uniprot.org/core/volume | "145"xsd:string |