| http://purl.uniprot.org/citations/10207026 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10207026 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10207026 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/10207026 | http://www.w3.org/2000/01/rdf-schema#comment | "NEDD8 is a ubiquitin-like molecule that can be covalently conjugated to a limited number of cellular proteins, such as Cdc53/cullin. We have previously reported that the C terminus of NEDD8 is efficiently processed to expose Gly-76, which is required for conjugation to target proteins. A combination of data base searches and polymerase chain reaction cloning was used to identify a cDNA encoding human UBA3, which is 38% identical to the yeast homologue, 22% identical to human UBA2, and 19% identical to the C-terminal region of human UBE1. The human UBA3 gene is located on chromosome 3p13 and gave rise to a 2.2-kilobase pair transcript that was detected in all tissues. Human UBA3 could be precipitated with glutathione S-transferase (GST)-NEDD8, but not with GST-ubiquitin or GST-sentrin-1. Moreover, human UBA3 could form a beta-mercaptoethanol-sensitive conjugate with NEDD8 in the presence of APP-BP1, a protein with sequence homology to the N-terminal half of ubiquitin-activating enzyme. We have also cloned human UBC12 and demonstrated that it could form a thiol ester linkage with NEDD8 in the presence of the activating enzyme complex. Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway should allow for a more detailed study of the role of NEDD8 modification in health and disease."xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.17.12036"xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.17.12036"xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/author | "Gong L."xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/author | "Gong L."xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/author | "Yeh E.T.H."xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/author | "Yeh E.T.H."xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/pages | "12036-12042"xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/pages | "12036-12042"xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/title | "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway."xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/title | "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway."xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10207026 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10207026 |
| http://purl.uniprot.org/citations/10207026 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10207026 |
| http://purl.uniprot.org/citations/10207026 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10207026 |
| http://purl.uniprot.org/citations/10207026 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10207026 |
| http://purl.uniprot.org/citations/10207026 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10207026 |