| http://purl.uniprot.org/citations/10210201 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10210201 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10210201 | http://www.w3.org/2000/01/rdf-schema#comment | "Gelsolin is an actin-binding protein (82 kDa) consisting of six repeated segments (S1-S6), each approximately 120 residues long. It interacts with phospholipids and we previously showed that phosphatidylinositol 4,5-bisphosphate promotes phosphorylation of gelsolin by the tyrosine kinase c-Src. We used a combination of different methods, such as thin-layer chromatography and anti-phosphotyrosine-agarose immunoprecipitation of phosphopeptides combined with matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) and post source decay (PSD) analysis, to identify the phosphorylation sites in gelsolin. The major phosphorylation site (Tyr438) was located in subdomain 4 (S4). Phosphorylation of gelsolin in the gelsolin-actin2 complex was inhibited by 90%. Gelsolin phosphorylation by c-Src in the presence of lysophosphatidic acid also revealed Tyr438 as the most prominent site. Additional minor sites were found using the anti-phosphotyrosine bead immunoprecipitation method followed by MALDI-MS and PSD analysis. These sites, representing approximately 5% of the total phosphate incorporation, were identified as Tyr59, Tyr382, Tyr576, and Tyr624. Based on these results we generated antibodies which specifically recognize Tyr438 phosphorylated gelsolin."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.org/dc/terms/identifier | "doi:10.1110/ps.8.1.234"xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.org/dc/terms/identifier | "doi:10.1110/ps.8.1.234"xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/author | "Demol H."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/author | "Demol H."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/author | "Goethals M."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/author | "Goethals M."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/author | "Van Damme J."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/author | "Van Damme J."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/author | "Vandekerckhove J."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/author | "Vandekerckhove J."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/author | "Gettemans J."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/author | "Gettemans J."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/author | "De Corte V."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/author | "De Corte V."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/name | "Protein Sci."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/name | "Protein Sci."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/pages | "234-241"xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/pages | "234-241"xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/title | "Identification of Tyr438 as the major in vitro c-Src phosphorylation site in human gelsolin: a mass spectrometric approach."xsd:string |
| http://purl.uniprot.org/citations/10210201 | http://purl.uniprot.org/core/title | "Identification of Tyr438 as the major in vitro c-Src phosphorylation site in human gelsolin: a mass spectrometric approach."xsd:string |