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http://purl.uniprot.org/citations/10212203http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10212203http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10212203http://www.w3.org/2000/01/rdf-schema#comment"After activation, agonist-occupied G protein-coupled receptors are phosphorylated by G protein-coupled receptor kinases and bind cytosolic beta-arrestins, which uncouple the receptors from their cognate G proteins. Recent studies on the beta2-adrenergic receptor have demonstrated that beta-arrestin also targets the receptors to clathrin-coated pits for subsequent internalization and activation of mitogen-activated protein kinases. We and others have previously shown that muscarinic acetylcholine receptors (mAChRs) of the m1, m3, and m4 subtype require functional dynamin to sequester into HEK-293 tsA201 cells, whereas m2 mAChRs sequester in a dynamin-independent manner. To investigate the role of beta-arrestin in mAChR sequestration, we determined the effect of overexpressing beta-arrestin-1 and the dominant-negative inhibitor of beta-arrestin-mediated receptor sequestration, beta-arrestin-1 V53D, on mAChR sequestration and function. Sequestration of m1, m3, and m4 mAChRs was suppressed by 60-75% in cells overexpressing beta-arrestin-1 V53D, whereas m2 mAChR sequestration was affected by less than 10%. In addition, overexpression of beta-arrestin-1 V53D as well as dynamin K44A significantly suppressed m1 mAChR-mediated activation of mitogen-activated protein kinases. Finally, we investigated whether mAChRs sequester into clathrin-coated vesicles by overexpressing Hub, a dominant-negative clathrin mutant. Although sequestration of m1, m3, and m4 mAChRs was inhibited by 50-70%, m2 mAChR sequestration was suppressed by less than 10%. We conclude that m1, m3, and m4 mAChRs expressed in HEK-293 tsA201 cells sequester into clathrin-coated vesicles in a beta-arrestin- and dynamin-dependent manner, whereas sequestration of m2 mAChRs in these cells is largely independent of these proteins."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.org/dc/terms/identifier"doi:10.1074/jbc.274.18.12333"xsd:string
http://purl.uniprot.org/citations/10212203http://purl.org/dc/terms/identifier"doi:10.1074/jbc.274.18.12333"xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/author"Schmidt M."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/author"Schmidt M."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/author"Voss M."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/author"Voss M."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/author"van Koppen C.J."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/author"van Koppen C.J."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/author"Jakobs K.H."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/author"Jakobs K.H."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/author"Nolte B."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/author"Nolte B."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/author"Voegler O."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/author"Voegler O."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/pages"12333-12338"xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/pages"12333-12338"xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/title"Regulation of muscarinic acetylcholine receptor sequestration and function by beta-arrestin."xsd:string
http://purl.uniprot.org/citations/10212203http://purl.uniprot.org/core/title"Regulation of muscarinic acetylcholine receptor sequestration and function by beta-arrestin."xsd:string
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