| http://purl.uniprot.org/citations/10216230 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10216230 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10216230 | http://www.w3.org/2000/01/rdf-schema#comment | "Tip1p is one of the major cell wall mannoproteins of Saccharomyces cerevisiae and is presumed to be synthesized as a glycosylphosphatidylinositol (GPI)-anchored form. We purified Tip1p from a glucanase extract of yeast cell walls and analyzed the sugar chain involved in the cell wall linkage. One mol of glucanase-extracted Tip1p contained 7.5 mol of glucose derived from glucan and 1 mol of ethanolamine, a component of the GPI anchor. One mol of the C-terminal peptide of Tip1p digested with Achromobacter protease I also contained 7.9 mol of glucose and 1 mol of ethanolamine. On the other hand, Tip1p contained no glucosamine, which is a component of the GPI anchor. The glucan-binding sugar chain of Tip1p was released by hydrazinolysis and isolated. This sugar chain contained ethanolamine with a free amino group and a glucose reducing end, but no mannose reducing end. Phosphodiesterase treatment eliminated the free amino group from this sugar chain, suggesting that a phosphodiester bond exists between the ethanolamine and the glucan remnant. These results indicate (1) the glucan-binding sugar chain of Tip1p is a GPI derivative, and (2) the GPI anchor is cleaved at the glycosyl moiety, and the resultant mannose reducing end is probably used to link Tip1p to cell wall glucan."xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0304-4165(99)00012-4"xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0304-4165(99)00012-4"xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/author | "Fujii T."xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/author | "Fujii T."xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/author | "Iimura Y."xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/author | "Iimura Y."xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/author | "Shimoi H."xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/author | "Shimoi H."xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/pages | "133-144"xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/pages | "133-144"xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/title | "Structure of the glucan-binding sugar chain of Tip1p, a cell wall protein of Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/title | "Structure of the glucan-binding sugar chain of Tip1p, a cell wall protein of Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/volume | "1427"xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://purl.uniprot.org/core/volume | "1427"xsd:string |
| http://purl.uniprot.org/citations/10216230 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10216230 |
| http://purl.uniprot.org/citations/10216230 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10216230 |
| http://purl.uniprot.org/citations/10216230 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10216230 |
| http://purl.uniprot.org/citations/10216230 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10216230 |