| http://purl.uniprot.org/citations/10224290 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10224290 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10224290 | http://www.w3.org/2000/01/rdf-schema#comment | "Alveolar macrophages (AMs) avidly bind and ingest unopsonized environmental particles and bacteria through scavenger-type receptors (SRs). AMs from mice with a genetic deletion of the major macrophage SR (types AI and AII; SR-/-) showed no decrease in particle binding compared with SR+/+ mice, suggesting that other SRs are involved. To identify these receptors, we generated a monoclonal antibody (mAb), PAL-1, that inhibits hamster AM binding of unopsonized particles (TiO2, Fe2O3, and latex beads; 66 +/- 5, 77 +/- 2, and 85 +/-2% inhibition, respectively, measured by flow cytometry). This antibody identifies a protein of approximately 70 kD on the AM surface (immunoprecipitation) that is expressed by AMs and other macrophages in situ. A cDNA clone encoding the mAb PAL-1-reactive protein isolated by means of COS cell expression was found to be 84 and 77% homologous to mouse and human scavenger receptor MARCO mRNA, respectively. Transfection of COS cells with MARCO cDNA conferred mAb-inhibitable TiO2 binding. Hamster MARCO also mediates AM binding of unopsonized bacteria (67 +/- 5 and 47 +/-4% inhibition of Escherichia coli and Staphylococcus aureus binding by mAb PAL-1). A polyclonal antibody to human MARCO identified the expected approximately 70-kD band on Western blots of lysates of normal bronchoalveolar lavage (BAL) cells (>90% AMs) and showed strong immunolabeling of human AMs in BAL cytocentrifuge preparations and within lung tissue specimens. In normal mouse AMs, the anti-MARCO mAb ED31 also showed immunoreactivity and inhibited binding of unopsonized particles (e.g., TiO2 approximately 40%) and bacteria. The novel function of binding unopsonized environmental dusts and pathogens suggests an important role for MARCO in the lungs' response to inhaled particles."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.org/dc/terms/identifier | "doi:10.1084/jem.189.9.1497"xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.org/dc/terms/identifier | "doi:10.1084/jem.189.9.1497"xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Kodama T."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Kodama T."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Suzuki H."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Suzuki H."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Qin G."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Qin G."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Kobzik L."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Kobzik L."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Tryggvason K."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Tryggvason K."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Paulauskis J.D."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Paulauskis J.D."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Al-Mutairi E."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Al-Mutairi E."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Imrich A."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Imrich A."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Koziel H."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Koziel H."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Palecanda A."xsd:string |
| http://purl.uniprot.org/citations/10224290 | http://purl.uniprot.org/core/author | "Palecanda A."xsd:string |