| http://purl.uniprot.org/citations/10225855 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10225855 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10225855 | http://www.w3.org/2000/01/rdf-schema#comment | "Motility of Helicobacter species has been shown to be essential for successful colonization of the host. We have investigated the organization of a flagellar export locus in Helicobacter pylori. A 7-kb fragment of the H. pylori CCUG 17874 genome was cloned and sequenced, revealing an operon comprising an open reading frame of unknown function (ORF03), essential housekeeping genes (ileS and murB), flagellar export genes (fliI and fliQ), and a homolog to a gene implicated in virulence factor transport in other pathogens (virB11). A promoter for this operon, showing similarity to the Escherichia coli sigma70 consensus, was identified by primer extension. Cotranscription of the genes in the operon was demonstrated by reverse transcription-PCR, and transcription of virB11, fliI, fliQ, and murB was detected in human or mouse biopsies obtained from infected hosts. The genetic organization of this locus was conserved in a panel of H. pylori clinical isolates. Engineered fliI and fliQ mutant strains were completely aflagellate and nonmotile, whereas a virB11 mutant still produced flagella. The fliI and fliQ mutant strains produced reduced levels of flagellin and the hook protein FlgE. Production of OMP4, a member of the outer membrane protein family identified in H. pylori 26695, was reduced in both the virB11 mutant and the fliI mutant, suggesting related functions of the virulence factor export protein (VirB11) and the flagellar export component (FliI)."xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.org/dc/terms/identifier | "doi:10.1128/iai.67.5.2060-2070.1999"xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.org/dc/terms/identifier | "doi:10.1128/iai.67.5.2060-2070.1999"xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/author | "O'Toole P.W."xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/author | "O'Toole P.W."xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/author | "Porwollik S."xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/author | "Porwollik S."xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/author | "Noonan B."xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/author | "Noonan B."xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/name | "Infect. Immun."xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/name | "Infect. Immun."xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/pages | "2060-2070"xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/pages | "2060-2070"xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/title | "Molecular characterization of a flagellar export locus of Helicobacter pylori."xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/title | "Molecular characterization of a flagellar export locus of Helicobacter pylori."xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/volume | "67"xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://purl.uniprot.org/core/volume | "67"xsd:string |
| http://purl.uniprot.org/citations/10225855 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10225855 |
| http://purl.uniprot.org/citations/10225855 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10225855 |
| http://purl.uniprot.org/citations/10225855 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10225855 |
| http://purl.uniprot.org/citations/10225855 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10225855 |