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http://purl.uniprot.org/citations/10225945http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10225945http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10225945http://www.w3.org/2000/01/rdf-schema#comment"We have identified a maize homologue of yeast MAD2, an essential component in the spindle checkpoint pathway that ensures metaphase is complete before anaphase begins. Combined immunolocalization of MAD2 and a recently cloned maize CENPC homologue indicates that MAD2 localizes to an outer domain of the prometaphase kinetochore. MAD2 staining was primarily observed on mitotic kinetochores that lacked attached microtubules; i.e., at prometaphase or when the microtubules were depolymerized with oryzalin. In contrast, the loss of MAD2 staining in meiosis was not correlated with initial microtubule attachment but was correlated with a measure of tension: the distance between homologous or sister kinetochores (in meiosis I and II, respectively). Further, the tension-sensitive 3F3/2 phosphoepitope colocalized, and was lost concomitantly, with MAD2 staining at the meiotic kinetochore. The mechanism of spindle assembly (discussed here with respect to maize mitosis and meiosis) is likely to affect the relative contributions of attachment and tension. We support the idea that MAD2 is attachment-sensitive and that tension stabilizes microtubule attachments."xsd:string
http://purl.uniprot.org/citations/10225945http://purl.org/dc/terms/identifier"doi:10.1083/jcb.145.3.425"xsd:string
http://purl.uniprot.org/citations/10225945http://purl.org/dc/terms/identifier"doi:10.1083/jcb.145.3.425"xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/author"Dawe R.K."xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/author"Dawe R.K."xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/author"Muszynski M.G."xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/author"Muszynski M.G."xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/author"Yu H.-G."xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/author"Yu H.-G."xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/name"J. Cell Biol."xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/name"J. Cell Biol."xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/pages"425-435"xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/pages"425-435"xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/title"The maize homologue of the cell cycle checkpoint protein MAD2 reveals kinetochore substructure and contrasting mitotic and meiotic localization patterns."xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/title"The maize homologue of the cell cycle checkpoint protein MAD2 reveals kinetochore substructure and contrasting mitotic and meiotic localization patterns."xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/volume"145"xsd:string
http://purl.uniprot.org/citations/10225945http://purl.uniprot.org/core/volume"145"xsd:string
http://purl.uniprot.org/citations/10225945http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10225945
http://purl.uniprot.org/citations/10225945http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10225945
http://purl.uniprot.org/citations/10225945http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/10225945
http://purl.uniprot.org/citations/10225945http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/10225945