| http://purl.uniprot.org/citations/10226032 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10226032 | http://www.w3.org/2000/01/rdf-schema#comment | "Cyclin-dependent kinases (Cdks) are fully active only when phosphorylated by a Cdk-activating kinase (CAK) [1]. Metazoan CAK is itself a Cdk, Cdk7, whereas the CAK of Saccharomyces cerevisiae is a distinct enzyme unrelated to Cdks [1]. The Mcs6-Mcs2 complex of Schizosaccharomyces pombe is a putative CAK related to the metazoan enzyme [2] [3]. Although the loss of Mcs6 is lethal, it results in a phenotype that is inconsistent with a failure to activate Cdc2, the major Cdk in S. pombe [3]. We therefore tested the ability of Csk1, a putative regulator of Mcs6 [4], to activate Cdk-cyclin complexes in vitro. Csk1 activated both the monomeric and the Mcs2-bound forms of Mcs6. Surprisingly, Csk1 also activated Cdc2 in complexes with either Cdc13 or Cig2 cyclins. When a double mutant carrying a csk1 deletion and a temperature-sensitive mcs6 allele was incubated at the restrictive temperature, Cdc2 was not activated and the cells underwent a cell division arrest prior to mitosis. Cdc2-cyclin complexes isolated from the arrested cells could be activated in vitro by recombinant CAK, whereas complexes from wild-type cells or either of the single mutants were refractory to activation. Thus, fission yeast contains two partially redundant CAKs: the Mcs6-Mcs2 complex and Csk1. Inactivation of both CAKs is necessary and sufficient to prevent Cdc2 activation and cause a cell-cycle arrest. Mcs6, which is essential, may therefore have required functions other than Cdk activation."xsd:string |
| http://purl.uniprot.org/citations/10226032 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0960-9822(99)80194-8"xsd:string |
| http://purl.uniprot.org/citations/10226032 | http://purl.uniprot.org/core/author | "Saiz J.E."xsd:string |
| http://purl.uniprot.org/citations/10226032 | http://purl.uniprot.org/core/author | "Lee K.M."xsd:string |
| http://purl.uniprot.org/citations/10226032 | http://purl.uniprot.org/core/author | "Barton W.A."xsd:string |
| http://purl.uniprot.org/citations/10226032 | http://purl.uniprot.org/core/author | "Fisher R.P."xsd:string |
| http://purl.uniprot.org/citations/10226032 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10226032 | http://purl.uniprot.org/core/name | "Curr Biol"xsd:string |
| http://purl.uniprot.org/citations/10226032 | http://purl.uniprot.org/core/pages | "441-444"xsd:string |
| http://purl.uniprot.org/citations/10226032 | http://purl.uniprot.org/core/title | "Cdc2 activation in fission yeast depends on Mcs6 and Csk1, two partially redundant Cdk-activating kinases (CAKs)."xsd:string |
| http://purl.uniprot.org/citations/10226032 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/10226032 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10226032 |
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| http://purl.uniprot.org/uniprot/P36615#attribution-AD85B00B7B29509E205358099447DBC2 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/10226032 |
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