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http://purl.uniprot.org/citations/10231327http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10231327http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10231327http://www.w3.org/2000/01/rdf-schema#comment"

Background

In the medical literature immunoglobulin (Ig)E-mediated sensitization to avocado is rarely reported. On the other hand, more than 50% of subjects having IgE-mediated natural rubber latex allergy are sensitized to avocado fruit as demonstrated by skin-prick testing and/or specific IgE measurements and about 10-20% report hypersensitivity reactions after ingesting avocado.

Objective

The underlying pathomechanism of latex-associated avocado allergy is still unknown. The conserved hevein domain of the major latex allergen prohevein (Hev b 6.01) is a ubiquitous chitin-binding protein structure that can be found in several plant proteins and may be responsible for the observed cross-reactivity between latex and avocado fruit.

Methods

Chitin-binding avocado proteins (CBAPs) were isolated by affinity-chromatography and their IgE-binding characteristics were studied by immunoblotting using the sera from 15 avocado-sensitized latex patients. Inhibition experiments using isolated hevein and CBAPs as inhibitor solutions were performed to study the immunological cross-reactivity between both protein species and to assess the role of the CBAPs as mediators in latex-associated avocado allergy.

Results

In 80% of avocado-sensitized subjects (n = 15), IgE antibodies directed against a 31-kDa allergen were detected by immunoblotting. This IgE-binding protein was identified by protein sequencing to be a class I endochitinase containing a hevein domain at the N-terminus. Purified native and digested (using simulated gastric fluid) endochitinase were able to completely block all avocado-specific IgE antibodies in six out of seven avocado patients.

Conclusions

Sensitization to endochitinase class I containing a hevein domain is the main underlying pathomechanism in latex-mediated avocado allergy."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.org/dc/terms/identifier"doi:10.1046/j.1365-2222.1999.00502.x"xsd:string
http://purl.uniprot.org/citations/10231327http://purl.org/dc/terms/identifier"doi:10.1046/j.1365-2222.1999.00502.x"xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Chen Z."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Chen Z."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Dunn M.J."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Dunn M.J."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Wheeler C.H."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Wheeler C.H."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Baur X."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Baur X."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Flagge A."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Flagge A."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Papenfuss F."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Papenfuss F."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Posch A."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Posch A."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Raulf-Heimsoth M."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/author"Raulf-Heimsoth M."xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/name"Clin. Exp. Allergy"xsd:string
http://purl.uniprot.org/citations/10231327http://purl.uniprot.org/core/name"Clin. Exp. Allergy"xsd:string
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