| http://purl.uniprot.org/citations/10321720 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10321720 | http://www.w3.org/2000/01/rdf-schema#comment | "Alpha2-adrenoceptor (alpha2-AR)-regulated binding of the labelled GTP analog, guanosine 5'-O-(3-[35S]thiotriphosphate) ([35S]GTPgammaS), to guanine-nucleotide-binding proteins (G proteins) was studied in human platelet membranes. Under optimal conditions, the potent alpha2-AR agonist, 5-bromo-6-(2-imidazolin-2-ylamino)-quinoxaline (UK 14304), increased the binding of [35S]GTPgammaS up to approximately 1.8 fold, with half-maximal increase at 60 nM and was competitively inhibited by the alpha2-AR antagonist Rauwolscine. The actions of both UK 14304 and Rauwolscine were modulated by monovalent and divalent cation levels, as well as by the concentrations of GDP. [35S]GTPgammaS binding induced by UK 14304 had a Kd value of 4.5 +/-0.3 nM and a Bmax value of 4.15 +/-0.40 pmol/mg protein. The rank order of potencies of adrenergic ligands tested in stimulating [3S]GTPgammaS binding and inhibiting forskolin-stimulated c-AMP accumulation was UK 14304> Guanabenz acetate> Oxymetazoline hydrochloride> B-HT 920 dihydrochloride> p-Aminoclonidine hydrochloride> Clonidine hydrochloride. The data presented indicate that enhancement of [35S]GTPgammaS binding by alpha2-AR in human platelet membranes provides a simple functional measure for receptor activation and can be used for determination of potencies and efficacies of ligands at the alpha2-AR."xsd:string |
| http://purl.uniprot.org/citations/10321720 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0024-3205(99)00074-0"xsd:string |
| http://purl.uniprot.org/citations/10321720 | http://purl.uniprot.org/core/author | "Borea P.A."xsd:string |
| http://purl.uniprot.org/citations/10321720 | http://purl.uniprot.org/core/author | "Campi F."xsd:string |
| http://purl.uniprot.org/citations/10321720 | http://purl.uniprot.org/core/author | "Gessi S."xsd:string |
| http://purl.uniprot.org/citations/10321720 | http://purl.uniprot.org/core/author | "Varani K."xsd:string |
| http://purl.uniprot.org/citations/10321720 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10321720 | http://purl.uniprot.org/core/name | "Life Sci"xsd:string |
| http://purl.uniprot.org/citations/10321720 | http://purl.uniprot.org/core/pages | "1403-1413"xsd:string |
| http://purl.uniprot.org/citations/10321720 | http://purl.uniprot.org/core/title | "Alpha2-adrenergic agonist modulation of [35S]GTPgammaS binding to guanine-nucleotide-binding-proteins in human platelet membranes."xsd:string |
| http://purl.uniprot.org/citations/10321720 | http://purl.uniprot.org/core/volume | "64"xsd:string |
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| http://purl.uniprot.org/uniprot/P18825 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10321720 |
| http://purl.uniprot.org/uniprot/P18089 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10321720 |
| http://purl.uniprot.org/uniprot/P08913 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10321720 |