| http://purl.uniprot.org/citations/10329453 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10329453 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10329453 | http://www.w3.org/2000/01/rdf-schema#comment | "Sialidase (EC 3.2.1.18) catalyzes the release of sialic acid from sialo-oligosaccharides, gangliosides, or sialo-glycoproteins. In this investigation, we cloned a novel cDNA for mouse brain sialidase and expressed the cDNA in COS-7 cells. This 1,699 bp cDNA codes for a 41.6 kDa protein consisting of 372 deduced amino acid residues. In COS-7 cells transiently transfected with the cDNA, a 250-fold increase was observed in specific activity toward 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid. Similarity searches of the nonredundant GenBank peptide sequence database by the PSI-BLAST program identified rat, hamster, human, and bacterial sialidases homologous to this mouse brain sialidase. Amino acid sequence identities to rat and hamster sialidases (84% and 77%, respectively) suggest that this form of sialidase is conserved in rodents. Sequence identities to human and mouse lysosomal sialidases (30% and 28%, respectively) indicate that the mouse brain sialidase is distinct from the lysosomal enzyme. Mouse brain sialidase has two amino acid sequence motifs common to bacterial sialidases: the 'F/YRIP' motif and the 'Asp-box' motif. The 'F/YRIP' motif is present near the N terminus while two 'Asp-box' motifs are present downstream."xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.1999.0698"xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.1999.0698"xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/author | "Gao L."xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/author | "Gao L."xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/author | "Yu R.K."xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/author | "Yu R.K."xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/author | "Zeng G."xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/author | "Zeng G."xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/author | "Fronda C.L."xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/author | "Fronda C.L."xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/pages | "727-731"xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/pages | "727-731"xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/title | "Molecular cloning and expression of mouse brain sialidase."xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/title | "Molecular cloning and expression of mouse brain sialidase."xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/volume | "258"xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://purl.uniprot.org/core/volume | "258"xsd:string |
| http://purl.uniprot.org/citations/10329453 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10329453 |
| http://purl.uniprot.org/citations/10329453 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10329453 |