| http://purl.uniprot.org/citations/10347202 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10347202 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10347202 | http://www.w3.org/2000/01/rdf-schema#comment | "We have isolated two novel Krüppel-like zinc finger proteins containing the evolutionarily conserved Krüppel-associated box (KRAB), KRAZ1 and KRAZ2, and demonstrated that they repress transcription when heterologously targeted to DNA. Their repression activity appeared to be mediated by the putative corepressor KAP-1 (KRAB-associated protein-1), because KRAZ1/2 bind to KAP-1, but KRAB mutants of KRAZ1/2 that are unable to interact with KAP-1 lack repression activity, and KAP-1 has intrinsic repressor activity and potentiates KRAZ1/2-mediated repression. We dissected the KAP-1 protein into a KRAB-interacting domain and a region necessary for repression. Using a mammalian two-hybrid assay, we further demonstrated that KAP-1 deletions lacking repression activity fused to the VP16 transactivation domain strongly activated transcription when coexpressed with KRAZ1. In contrast, VP16-KAP-1 fusions retaining repression activity resulted in repression. These results provide the first evidence that KAP-1 functionally interacts with KRAB in mammalian cells and seems to exert repressor activity in the DNA-bound KRAB-KAP-1 complex, and they further support the hypothesis that KAP-1 functions as a corepressor for the large class of KRAB-containing zinc finger proteins."xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.23.16412"xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.23.16412"xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/author | "Shimizu A."xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/author | "Shimizu A."xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/author | "Matsuda E."xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/author | "Matsuda E."xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/author | "Agata Y."xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/author | "Agata Y."xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/pages | "16412-16422"xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/pages | "16412-16422"xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/title | "Two novel Kruppel-associated box-containing zinc-finger proteins, KRAZ1 and KRAZ2, repress transcription through functional interaction with the corepressor KAP-1 (TIF1beta/KRIP-1)."xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/title | "Two novel Kruppel-associated box-containing zinc-finger proteins, KRAZ1 and KRAZ2, repress transcription through functional interaction with the corepressor KAP-1 (TIF1beta/KRIP-1)."xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10347202 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10347202 |
| http://purl.uniprot.org/citations/10347202 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10347202 |
| http://purl.uniprot.org/citations/10347202 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10347202 |
| http://purl.uniprot.org/citations/10347202 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10347202 |