| http://purl.uniprot.org/citations/10358090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10358090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10358090 | http://www.w3.org/2000/01/rdf-schema#comment | "We characterized type 3 ryanodine receptor (RyR3) purified from rabbit diaphragm by immunoaffinity chromatography using a specific antibody. The purified receptor was free from 12-kDa FK506-binding protein, although it retained the ability to bind 12-kDa FK506-binding protein. Negatively stained images of RyR3 show a characteristic rectangular structure that was indistinguishable from RyR1. The location of the D2 segment, which exists uniquely in the RyR1 isoform, was determined as the region around domain 9 close to the corner of the square-shaped assembly, with use of D2-directed antibody as a probe. The RyR3 homotetramer had a single class of high affinity [3H]ryanodine-binding sites with a stoichiometry of 1 mol/mol. In planar lipid bilayers, RyR3 displayed cation channel activity that was modulated by several ligands including Ca2+, Mg2+, caffeine, and ATP, which is consistent with [3H]ryanodine binding activity. RyR3 showed a slightly larger unit conductance and a longer mean open time than RyR1. Whereas RyR1 showed two classes of channel activity with distinct open probabilities (Po), RyR3 displayed a homogeneous and steeply Ca2+-dependent activity with Po approximately 1. RyR3 was more steeply affected in the channel activity by sulfhydryl-oxidizing and -reducing reagents than RyR1, suggesting that the channel activity of RyR3 may be transformed more precipitously by the redox state. This is also a likely explanation for the difference in the Ca2+ dependence of RyR3 between [3H]ryanodine binding and channel activity."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.24.17297"xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.24.17297"xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Kobayashi M."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Kobayashi M."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Ogawa Y."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Ogawa Y."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Otsuka K."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Otsuka K."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Oguchi K."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Oguchi K."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Murayama T."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Murayama T."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Oba T."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Oba T."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Katayama E."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Katayama E."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Oyamada H."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/author | "Oyamada H."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10358090 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |