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http://purl.uniprot.org/citations/10381338http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10381338http://www.w3.org/2000/01/rdf-schema#comment"The present paper describes a rapid and systematic method for semi-quantitative analysis of the expression of sialyltransferase genes. So far, fifteen sialyltransferase cDNAs have been cloned from mice. Most of these genes are expressed in developmental stage-dependent and/or tissue-specific manners, and the expression levels of some of them are too low to detect on Northern blot analysis. To resolve how each sialyltransferase contribute to synthesize sialylglycoconjugates, it is necessary to establish the method for quantification of gene expression levels of these fifteen sialyltransferases. Therefore, we developed a competitive PCR-based method for analyzing the quantitative relationship of the gene expression of fifteen sialyltransferases. Using this method, we can investigate the levels of gene expression of sialyltransferases in various cell lines and various tissues of mice, and can accurately determine their expression levels."xsd:string
http://purl.uniprot.org/citations/10381338http://purl.org/dc/terms/identifier"doi:10.1006/bbrc.1999.0794"xsd:string
http://purl.uniprot.org/citations/10381338http://purl.uniprot.org/core/author"Nakagawa T."xsd:string
http://purl.uniprot.org/citations/10381338http://purl.uniprot.org/core/author"Hamamoto T."xsd:string
http://purl.uniprot.org/citations/10381338http://purl.uniprot.org/core/author"Tsuji S."xsd:string
http://purl.uniprot.org/citations/10381338http://purl.uniprot.org/core/author"Takashima S."xsd:string
http://purl.uniprot.org/citations/10381338http://purl.uniprot.org/core/author"Tachida Y."xsd:string
http://purl.uniprot.org/citations/10381338http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10381338http://purl.uniprot.org/core/name"Biochem Biophys Res Commun"xsd:string
http://purl.uniprot.org/citations/10381338http://purl.uniprot.org/core/pages"23-27"xsd:string
http://purl.uniprot.org/citations/10381338http://purl.uniprot.org/core/title"Quantitative analysis of expression of mouse sialyltransferase genes by competitive PCR."xsd:string
http://purl.uniprot.org/citations/10381338http://purl.uniprot.org/core/volume"260"xsd:string
http://purl.uniprot.org/citations/10381338http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10381338
http://purl.uniprot.org/citations/10381338http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/10381338
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http://purl.uniprot.org/uniprot/#_A0A1L1SUM8-mappedCitation-10381338http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/10381338
http://purl.uniprot.org/uniprot/#_A0A087WRA0-mappedCitation-10381338http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/10381338
http://purl.uniprot.org/uniprot/#_A0A0A6YVT0-mappedCitation-10381338http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/10381338