| http://purl.uniprot.org/citations/10393106 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10393106 | http://www.w3.org/2000/01/rdf-schema#comment | "Intestinal trefoil factor (ITF) is expressed selectively in intestinal goblet cells. Previous studies of the rat ITF gene identified one cis-regulatory element, designated the goblet-cell-response element (GCRE), present in the proximal region of the promoter. To identify additional cis-regulatory elements responsible for goblet-cell-specific expression, a DNA fragment containing 6353 bp of the 5'-flanking region of the mouse ITF gene was cloned and its promoter activity was examined extensively. In human and murine intestinal-derived cell lines (LS174T and CMT-93), the luciferase activities of a 6.3-kb construct were 5- and 2-fold greater than the smaller 1.8-kb construct, respectively. In contrast, the activity in non-intestinal cell lines (HepG2 and HeLa) was 2-4-fold lower than the smaller construct. In the region downstream from the 1.8-kb position, strong luciferase activities in LS174T and HepG2 cells were observed using a 201-bp construct. Interestingly, increased activity was almost completely suppressed in cells transfected with a 391-bp construct. Detailed analyses of this region revealed the existence of a 11-bp positive regulatory element (-181 to -170; ACCTCTTCCTG) and a 9-bp negative regulatory element (-208 to -200; ATTGACAGA) in addition to the GCRE. All three elements were well conserved among human, rat and mouse ITF gene promoters. In addition, a mutant 1.8-kb construct in which the negative regulatory region was deleted yielded the same approximate luciferase activity as a 6.3-kb construct, suggesting binding of a goblet-cell-specific silencer inhibitor (SI) between -6.3 and -1.8 kb. The SI present in goblet cells may block the silencers' binding to the pre-initiation complex and allow increased transcriptional activity driven by specific and non-specific enhancers. High-level expression of the mouse ITF gene specifically in intestinal goblet cells may be achieved through the combined effects of these regulatory elements."xsd:string |
| http://purl.uniprot.org/citations/10393106 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3410461"xsd:string |
| http://purl.uniprot.org/citations/10393106 | http://purl.uniprot.org/core/author | "Itoh H."xsd:string |
| http://purl.uniprot.org/citations/10393106 | http://purl.uniprot.org/core/author | "Inoue N."xsd:string |
| http://purl.uniprot.org/citations/10393106 | http://purl.uniprot.org/core/author | "Podolsky D.K."xsd:string |
| http://purl.uniprot.org/citations/10393106 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10393106 | http://purl.uniprot.org/core/name | "Biochem J"xsd:string |
| http://purl.uniprot.org/citations/10393106 | http://purl.uniprot.org/core/pages | "461-472"xsd:string |
| http://purl.uniprot.org/citations/10393106 | http://purl.uniprot.org/core/title | "Goblet-cell-specific transcription of mouse intestinal trefoil factor gene results from collaboration of complex series of positive and negative regulatory elements."xsd:string |
| http://purl.uniprot.org/citations/10393106 | http://purl.uniprot.org/core/volume | "341"xsd:string |
| http://purl.uniprot.org/citations/10393106 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10393106 |
| http://purl.uniprot.org/citations/10393106 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10393106 |
| http://purl.uniprot.org/uniprot/#_Q62395-mappedCitation-10393106 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10393106 |
| http://purl.uniprot.org/uniprot/Q62395 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10393106 |