| http://purl.uniprot.org/citations/10393239 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10393239 | http://www.w3.org/2000/01/rdf-schema#comment | "The regulation of the rat fatty acid synthase gene by mediators such as diet, hormones, cAMP, sterols or retinoic acid is controlled by three NF-Y binding sites. All three sites have a neighbouring Sp1-binding GC-box. This NF-Y/Sp1 motif is conserved in the FAS promoters of rat, human, goose and chicken. We have previously shown cooperative binding of NF-Y and Sp1 to the promoter region at -500 coincident with a diet-induced DNAse I-hypersensitive site. Here, we show an in-vivo interaction of NF-YA with Sp1 using the yeast two-hybrid system. The interacting domains are located between amino acids 55 and 139 of the NF-Y subunit NF-YA and between amino acids 139 and 344 of Sp1. In addition, we show by co-immunoprecipitation direct interaction of NF-Y subunit NF-YA with Sp1 in extracts of rat hepatoma cells H4IIE. Furthermore, we demonstrate by the GST pull-down assay that NF-YA interacts physically with Sp1 in-vitro in the absence of DNA. Therefore, NF-Y can be added to the list of transcription factors interacting with Sp1."xsd:string |
| http://purl.uniprot.org/citations/10393239 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0378-1119(99)00180-8"xsd:string |
| http://purl.uniprot.org/citations/10393239 | http://purl.uniprot.org/core/author | "Schweizer M."xsd:string |
| http://purl.uniprot.org/citations/10393239 | http://purl.uniprot.org/core/author | "Wolf S.S."xsd:string |
| http://purl.uniprot.org/citations/10393239 | http://purl.uniprot.org/core/author | "Roder K."xsd:string |
| http://purl.uniprot.org/citations/10393239 | http://purl.uniprot.org/core/author | "Larkin K.J."xsd:string |
| http://purl.uniprot.org/citations/10393239 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10393239 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/10393239 | http://purl.uniprot.org/core/pages | "61-69"xsd:string |
| http://purl.uniprot.org/citations/10393239 | http://purl.uniprot.org/core/title | "Interaction between the two ubiquitously expressed transcription factors NF-Y and Sp1."xsd:string |
| http://purl.uniprot.org/citations/10393239 | http://purl.uniprot.org/core/volume | "234"xsd:string |
| http://purl.uniprot.org/citations/10393239 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10393239 |
| http://purl.uniprot.org/citations/10393239 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10393239 |
| http://purl.uniprot.org/uniprot/P08047#attribution-B2D39161C786B72895E19055A0F26D69 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/10393239 |
| http://purl.uniprot.org/uniprot/P08047#attribution-BF1605048219E3C4E28B2AE9BEA34BB1 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/10393239 |
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| http://purl.uniprot.org/uniprot/#_E0CY40-mappedCitation-10393239 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10393239 |
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| http://purl.uniprot.org/uniprot/#_A0A8L2Q8B5-mappedCitation-10393239 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10393239 |
| http://purl.uniprot.org/uniprot/#_A0A8I5Y9Y4-mappedCitation-10393239 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10393239 |
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| http://purl.uniprot.org/uniprot/#_F6TWX0-mappedCitation-10393239 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10393239 |
| http://purl.uniprot.org/uniprot/#_F7DFJ2-mappedCitation-10393239 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10393239 |