| http://purl.uniprot.org/citations/10402461 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10402461 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10402461 | http://www.w3.org/2000/01/rdf-schema#comment | "Expression cloning from a cDNA library prepared from a mutant CHO cell line with Golgi-specific resistance to Brefeldin A (BFA) identified a novel 206-kD protein with a Sec7 domain termed GBF1 for Golgi BFA resistance factor 1. Overexpression of GBF1 allowed transfected cells to maintain normal Golgi morphology and grow in the presence of BFA. Golgi-enriched membrane fractions from such transfected cells displayed normal levels of ADP ribosylation factors (ARFs) activation and coat protein recruitment that were, however, BFA resistant. Hexahistidine-tagged-GBF1 exhibited BFA-resistant guanine nucleotide exchange activity that appears specific towards ARF5 at physiological Mg2+concentration. Characterization of cDNAs recovered from the mutant and wild-type parental lines established that transcripts in these cells had identical sequence and, therefore, that GBF1 was naturally BFA resistant. GBF1 was primarily cytosolic but a significant pool colocalized to a perinuclear structure with the beta-subunit of COPI. Immunogold labeling showed highest density of GBF1 over Golgi cisternae and significant labeling over pleiomorphic smooth vesiculotubular structures. The BFA-resistant nature of GBF1 suggests involvement in retrograde traffic."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.146.1.71"xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.146.1.71"xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Berger S.J."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Berger S.J."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Melancon P."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Melancon P."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Dahan S."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Dahan S."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Claude A."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Claude A."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Armold A.D."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Armold A.D."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Kuziemsky C.E."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Kuziemsky C.E."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Sullivan E.M."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Sullivan E.M."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Yan J.-P."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Yan J.-P."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Zhao B.-P."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/author | "Zhao B.-P."xsd:string |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10402461 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |