| http://purl.uniprot.org/citations/10409727 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10409727 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10409727 | http://www.w3.org/2000/01/rdf-schema#comment | "The estrogen receptor (ER) regulates the expression of target genes in a ligand-dependent manner. The ligand-dependent activation function AF-2 of the ER is located in the ligand binding domain (LBD), while the N-terminal A/B domain (AF-1) functions in a ligand-independent manner when isolated from the LBD. AF-1 and AF-2 exhibit cell type and promoter context specificity. Furthermore, the AF-1 activity of the human ERalpha (hERalpha) is enhanced through phosphorylation of the Ser(118) residue by mitogen-activated protein kinase (MAPK). From MCF-7 cells, we purified and cloned a 68-kDa protein (p68) which interacted with the A/B domain but not with the LBD of hERalpha. Phosphorylation of hERalpha Ser(118) potentiated the interaction with p68. We demonstrate that p68 enhanced the activity of AF-1 but not AF-2 and the estrogen-induced as well as the anti-estrogen-induced transcriptional activity of the full-length ERalpha in a cell-type-specific manner. However, it did not potentiate AF-1 or AF-2 of ERbeta, androgen receptor, retinoic acid receptor alpha, or mineralocorticoid receptor. We also show that the RNA helicase activity previously ascribed to p68 is dispensable for the ERalpha AF-1 coactivator activity and that p68 binds to CBP in vitro. Furthermore, the interaction region for p68 in the ERalpha A/B domain was essential for the full activity of hERalpha AF-1. Taken together, these findings show that p68 acts as a coactivator specific for the ERalpha AF-1 and strongly suggest that the interaction between p68 and the hERalpha A/B domain is regulated by MAPK-induced phosphorylation of Ser(118)."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.19.8.5363"xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.19.8.5363"xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Kato S."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Kato S."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Kobayashi Y."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Kobayashi Y."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Hashimoto S."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Hashimoto S."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Maruyama K."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Maruyama K."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Endoh H."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Endoh H."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Goto M."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Goto M."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Tai H."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Tai H."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Metzger D."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Metzger D."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Yanagisawa J."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Yanagisawa J."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Masuhiro Y."xsd:string |
| http://purl.uniprot.org/citations/10409727 | http://purl.uniprot.org/core/author | "Masuhiro Y."xsd:string |