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| http://purl.uniprot.org/citations/10409768 | http://www.w3.org/2000/01/rdf-schema#comment | "During murine spermatogenesis, beginning in late pachytene spermatocytes, the beta4-galactosyltransferase-I (beta4GalT-I) gene is transcribed from a male germ cell-specific start site. We had shown previously that a 796-bp genomic fragment that flanks the germ cell start site and contains two putative CRE (cyclic AMP-responsive element)-like motifs directs correct male germ cell expression of the beta-galactosidase reporter gene in late pachytene spermatocytes and round spermatids of transgenic mice (N. L. Shaper, A. Harduin-Lepers, and J. H. Shaper, J. Biol. Chem. 269:25165-25171, 1994). We now report that in vivo expression of beta4GalT-I in developing male germ cells requires an essential and previously undescribed 14-bp regulatory element (5'-GCCGGTTTCCTAGA-3') that is distinct from the two CRE-like sequences. This cis element is located 16 bp upstream of the germ cell-specific start site and binds a male germ cell protein that we have termed TASS-1 (transcriptional activator in late pachytene spermatocytes and round spermatids 1). The presence of the Ets signature binding motif 5'-GGAA-3' on the bottom strand of the TASS-1 sequence (underlined sequence) suggests that TASS-1 is a novel member of the Ets family of transcription factors. Additional transgenic analyses established that an 87-bp genomic fragment containing the TASS-1 regulatory element was sufficient for correct germ cell-specific expression of the beta-galactosidase reporter gene. Furthermore, when the TASS-1 motif was mutated by transversion, within the context of the original 796-bp fragment, transgene expression was reduced 12-to 35-fold in vivo."xsd:string |
| http://purl.uniprot.org/citations/10409768 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.19.8.5823"xsd:string |
| http://purl.uniprot.org/citations/10409768 | http://purl.uniprot.org/core/author | "Shaper J.H."xsd:string |
| http://purl.uniprot.org/citations/10409768 | http://purl.uniprot.org/core/author | "Shaper N.L."xsd:string |
| http://purl.uniprot.org/citations/10409768 | http://purl.uniprot.org/core/author | "Charron M."xsd:string |
| http://purl.uniprot.org/citations/10409768 | http://purl.uniprot.org/core/author | "Rajput B."xsd:string |
| http://purl.uniprot.org/citations/10409768 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10409768 | http://purl.uniprot.org/core/name | "Mol Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/10409768 | http://purl.uniprot.org/core/pages | "5823-5832"xsd:string |
| http://purl.uniprot.org/citations/10409768 | http://purl.uniprot.org/core/title | "A novel 14-base-pair regulatory element is essential for in vivo expression of murine beta4-galactosyltransferase-I in late pachytene spermatocytes and round spermatids."xsd:string |
| http://purl.uniprot.org/citations/10409768 | http://purl.uniprot.org/core/volume | "19"xsd:string |
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