| http://purl.uniprot.org/citations/10415121 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10415121 | http://www.w3.org/2000/01/rdf-schema#comment | "Alkyl-dihydroxyacetonephosphate synthase, a peroxisomal enzyme involved in the biosynthesis of ether phospholipids, is synthesized with a cleavable N-terminal presequence containing the peroxisomal targeting signal type 2. The human alkyl-dihydroxyacetonephosphate synthase precursor produced in vitro or expressed in Escherichia coli could be processed to a lower molecular weight protein by incubation at 37 degrees C with a guinea pig liver fraction, enriched in mitochondria, lysosomes, and peroxisomes. This lower molecular weight protein was identified as the mature human alkyl-dihydroxyacetonephosphate synthase by radiosequencing, indicating that the processing protease is present in this organellar fraction. Characterization of the processing protease indicated that it is a cysteine protease with a pH optimum of 6.5. Furthermore, it was demonstrated that exogenously added pre-alkyl-dihydroxyacetonephosphate synthase was imported and processed in purified peroxisomes in vitro. Processing of alkyl-dihydroxyacetonephosphate synthase did not increase the activity of the enzyme. This indicates that the presence of the presequence does not affect the activity of the enzyme."xsd:string |
| http://purl.uniprot.org/citations/10415121 | http://purl.org/dc/terms/identifier | "doi:10.1006/abbi.1999.1281"xsd:string |
| http://purl.uniprot.org/citations/10415121 | http://purl.uniprot.org/core/author | "van den Bosch H."xsd:string |
| http://purl.uniprot.org/citations/10415121 | http://purl.uniprot.org/core/author | "Biermann J."xsd:string |
| http://purl.uniprot.org/citations/10415121 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10415121 | http://purl.uniprot.org/core/name | "Arch Biochem Biophys"xsd:string |
| http://purl.uniprot.org/citations/10415121 | http://purl.uniprot.org/core/pages | "139-146"xsd:string |
| http://purl.uniprot.org/citations/10415121 | http://purl.uniprot.org/core/title | "In vitro processing of the human alkyl-dihydroxyacetonephosphate synthase precursor."xsd:string |
| http://purl.uniprot.org/citations/10415121 | http://purl.uniprot.org/core/volume | "368"xsd:string |
| http://purl.uniprot.org/citations/10415121 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10415121 |
| http://purl.uniprot.org/citations/10415121 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10415121 |
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| http://purl.uniprot.org/uniprot/#_O00116-mappedCitation-10415121 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10415121 |
| http://purl.uniprot.org/uniprot/O00116 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10415121 |
| http://purl.uniprot.org/uniprot/O15228 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10415121 |