| http://purl.uniprot.org/citations/10430621 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10430621 | http://www.w3.org/2000/01/rdf-schema#comment | "Mice deficient in various mismatch repair (MMR) enzymes were examined to determine whether this repair pathway is involved in antibody class switch recombination. Splenic B cells from mice deficient in Msh2, Mlh1, Pms2, or Mlh1 and Pms2 were stimulated in culture with lipopolysaccharide (LPS) to induce immunoglobulin (Ig)G2b and IgG3, LPS and interleukin (IL)-4 to induce IgG1, or LPS, anti-delta-dextran, IL-4, IL-5, and transforming growth factor (TGF)-beta1 to induce IgA. After 4 d in culture, cells were surface stained for IgM and non-IgM isotypes and analyzed by FACS((R)). B cells from MMR-deficient mice show a 35-75% reduction in isotype switching, depending on the isotype and on the particular MMR enzyme missing. IgG2b is the most affected, reduced by 75% in Mlh1-deficient animals. The switching defect is not due to a lack of maturation of the B cells, as purified IgM(+)IgD(+) B cells show the same reduction. MMR deficiency had no effect on cell proliferation, viability, or apoptosis, as detected by [(3)H]thymidine incorporation and by propidium iodide staining. The reduction in isotype switching was demonstrated to be at the level of DNA recombination by digestion-circularization polymerase chain reaction (DC-PCR). A model of the potential role for MMR enzymes in class switch recombination is presented."xsd:string |
| http://purl.uniprot.org/citations/10430621 | http://purl.org/dc/terms/identifier | "doi:10.1084/jem.190.3.323"xsd:string |
| http://purl.uniprot.org/citations/10430621 | http://purl.uniprot.org/core/author | "Kucherlapati R."xsd:string |
| http://purl.uniprot.org/citations/10430621 | http://purl.uniprot.org/core/author | "Schrader C.E."xsd:string |
| http://purl.uniprot.org/citations/10430621 | http://purl.uniprot.org/core/author | "Stavnezer J."xsd:string |
| http://purl.uniprot.org/citations/10430621 | http://purl.uniprot.org/core/author | "Edelmann W."xsd:string |
| http://purl.uniprot.org/citations/10430621 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10430621 | http://purl.uniprot.org/core/name | "J Exp Med"xsd:string |
| http://purl.uniprot.org/citations/10430621 | http://purl.uniprot.org/core/pages | "323-330"xsd:string |
| http://purl.uniprot.org/citations/10430621 | http://purl.uniprot.org/core/title | "Reduced isotype switching in splenic B cells from mice deficient in mismatch repair enzymes."xsd:string |
| http://purl.uniprot.org/citations/10430621 | http://purl.uniprot.org/core/volume | "190"xsd:string |
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