| http://purl.uniprot.org/citations/10430890 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10430890 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10430890 | http://www.w3.org/2000/01/rdf-schema#comment | "Elongin is a heterotrimeric transcription elongation factor composed of subunits A, B, and C in mammals. Elongin A and C are F-box-containing and SKP1 homologue proteins, respectively, and are therefore of interest for their potential roles in cell cycle-dependent proteolysis. Mammalian elongin C interacts with both elongin A and elongin B, as well as with the von Hippel-Lindau tumor suppressor protein VHL. To investigate the corresponding interactions in yeast, we have utilized NMR spectroscopy combined with ultracentrifugal sedimentation experiments to examine complexes of yeast elongin C (Elc1) with yeast elongin A (Ela1) and two peptides from homologous regions of Ela1 and human VHL. Elc1 alone is a homotetramer composed of subunits with a structured N-terminal region and a dynamically unstable C-terminal region. Binding of a peptide fragment of the Elc1-interaction domain of Ela1 or with a homologous peptide from VHL promotes folding of the C-terminal region of Elc1 into two regular helical structures and dissociates Elc1 into homodimers. Moreover, analysis of the complex of Elc1 with the full Elc1-interaction domain of Ela1 reveals that the Elc1 homodimer is dissociated to preferentially form an Ela1/Elc1 heterodimer. Thus, elongin C is found to oligomerize in solution and to undergo significant structural rearrangements upon binding of two different partner proteins. These results suggest a structural basis for the interaction of an F-box-containing protein with a SKP1 homologue and the modulation of this interaction by the tumor suppressor VHL."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.96.16.9033"xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.96.16.9033"xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Arrowsmith C.H."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Arrowsmith C.H."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Edwards A.M."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Edwards A.M."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Koth C.M."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Koth C.M."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Botuyan M.V."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Botuyan M.V."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Conaway J.W."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Conaway J.W."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Conaway R.C."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Conaway R.C."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Chakrabartty A."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Chakrabartty A."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Chazin W.J."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Chazin W.J."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Mer G."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/author | "Mer G."xsd:string |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10430890 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |