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http://purl.uniprot.org/citations/10436389http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10436389http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10436389http://www.w3.org/2000/01/rdf-schema#comment"

Background

We have previously described a cDNA (clone Bt6) encoding a novel allergen from Blomia tropicalis, which showed sequence similarities to the FABP/P2/cellular retinoic acid binding protein/cellular retinol binding protein, a family of cytosolic lipid transport proteins (cLTPs). This work was planned to better characterize this allergen to which the official name Blo t 13 had been assigned.

Methods

Fluorescence-based lipid ligand binding assays and secondary structure analysis by circular dicroism were carry out using recombinant Blo t 13 (rBlo t 13) protein. Structural predictions and molecular modelling were performed based on the amino acid sequence inferred from the open reading frame of Bt6 cDNA sequence.

Results

rBlot t 13 binds the natural fluorescent fatty acid cis-parinaric acid and oleic acid by competition, but not retinol, retinoic acid, cholesterol, dansylated or anthroxylated fatty acids such as dansyl-DL-aminocaprylic acid and 12-(9-anthroyloxy)-stereate. Circular dichroism analysis indicated that rBlo t 13 comprises 45% beta-sheet and 13% alpha-helix. The amino acid sequence of Blo t 13 modelled well to known crystal structures of cLTPs providing a tertiary structural model comprising ten beta-strands organized into two beta-sheets, and two short alpha-helices.

Conclusion

Blo t 13 is a fatty acid-specific member of the beta-rich cLTP family of proteins."xsd:string
http://purl.uniprot.org/citations/10436389http://purl.org/dc/terms/identifier"doi:10.1159/000024193"xsd:string
http://purl.uniprot.org/citations/10436389http://purl.org/dc/terms/identifier"doi:10.1159/000024193"xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/author"Kennedy M.W."xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/author"Kennedy M.W."xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/author"Jimenez S."xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/author"Jimenez S."xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/author"Caraballo L."xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/author"Caraballo L."xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/author"Puerta L."xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/author"Puerta L."xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/name"Int. Arch. Allergy Immunol."xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/name"Int. Arch. Allergy Immunol."xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/pages"181-184"xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/pages"181-184"xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/title"Structural and ligand binding analysis of recombinant Blo t 13 allergen from Blomia tropicalis mite, a fatty acid binding protein."xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/title"Structural and ligand binding analysis of recombinant Blo t 13 allergen from Blomia tropicalis mite, a fatty acid binding protein."xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/volume"119"xsd:string
http://purl.uniprot.org/citations/10436389http://purl.uniprot.org/core/volume"119"xsd:string
http://purl.uniprot.org/citations/10436389http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10436389
http://purl.uniprot.org/citations/10436389http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10436389
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