| http://purl.uniprot.org/citations/10455127 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10455127 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10455127 | http://www.w3.org/2000/01/rdf-schema#comment | "Digestion with trypsin of purified His-tagged NhaA in a solution of dodecyl maltoside yields two fragments at alkaline pH but only one fragment at acidic pH. Determination of the amino acid sequence of the N terminus of the cleavage products show that the pH-sensitive cleavage site of NhaA, both in isolated everted membrane vesicles as well as in the pure protein in detergent, is Lys-249 in loop VIII-IX, which connects transmembrane segment VIII to IX. Interestingly, the two polypeptide products of the split antiporter remain complexed and co-purify on Ni(2+)-NTA column. Loop VIII-IX has also been found to play a role in the pH regulation of NhaA; three mutations introduced into the loop shift the pH profile of the Na(+)/H(+) antiporter activity as measured in everted membrane vesicles. An insertion mutation introducing Ile-Glu-Gly between residues Lys-249 and Arg-250 (K249-IEG-R250) and Cys replacement of either Val-254 (V254C) or Glu-241 (E241C) cause acidic shift of the pH profile of the antiporter by 0.5, 1, and 0.3 pH units, respectively. Interestingly, the double mutant E241C/V254C introduces a basic shift of more than 1 pH unit with respect to the single mutation V254C. Taken together these results imply the involvement of loop VIII-IX in the pH-induced conformational change, which leads to activation of NhaA at alkaline pH."xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.35.24617"xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.35.24617"xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/author | "Padan E."xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/author | "Padan E."xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/author | "Gerchman Y."xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/author | "Gerchman Y."xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/author | "Rimon A."xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/author | "Rimon A."xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/pages | "24617-24624"xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/pages | "24617-24624"xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/title | "A pH-dependent conformational change of NhaA Na(+)/H(+) antiporter of Escherichia coli involves loop VIII-IX, plays a role in the pH response of the protein, and is maintained by the pure protein in dodecyl maltoside."xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/title | "A pH-dependent conformational change of NhaA Na(+)/H(+) antiporter of Escherichia coli involves loop VIII-IX, plays a role in the pH response of the protein, and is maintained by the pure protein in dodecyl maltoside."xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10455127 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10455127 |
| http://purl.uniprot.org/citations/10455127 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10455127 |
| http://purl.uniprot.org/citations/10455127 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10455127 |
| http://purl.uniprot.org/citations/10455127 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10455127 |