| http://purl.uniprot.org/citations/10456937 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10456937 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10456937 | http://www.w3.org/2000/01/rdf-schema#comment | "beta-Defensins are cationic peptides with broad-spectrum antimicrobial activity that may play a role in mucosal defenses of several organs. They have been isolated in several species, and in humans, two beta-defensins have been identified. Here, we report the identification of two genes encoding beta-defensin homologues in the rat. Partial cDNAs were found by searching the expressed-sequence-tag database, and primers were designed to generate full-length mRNA coding sequences. One gene was highly similar to the human beta-defensin-1 (HBD-1) gene and mouse beta-defensin-1 gene at both the nucleic acid and amino acid levels and was termed rat beta-defensin-1 (RBD-1). The other gene, named RBD-2, was homologous to the HBD-2 and bovine tracheal antimicrobial peptide (TAP) genes. The predicted prepropeptides were strongly cationic, were 69 and 63 residues in length for RBD-1 and RBD-2, respectively, and contained the six-cysteine motif characteristic of beta-defensins. The beta-defensin genes mapped closely on rat chromosome 16 and were closely linked to the alpha-defensins genes, suggesting that they are part of a gene cluster, similar to the organization reported for humans. Northern blot analysis showed that both RBD-1 and RBD-2 mRNA transcripts were approximately 0.5 kb in length; RBD-1 mRNA was abundantly transcribed in the rat kidney, while RBD-2 was prevalent in the lung. Reverse transcription-PCR indicated that RBD-1 and RBD-2 mRNAs were distributed in a variety of other tissues. In the lung, RBD-1 mRNA expression localized to the tracheal epithelium while RBD-2 was expressed in alveolar type II cells. In conclusion, we characterized two novel beta-defensin homologues in the rat. The rat may be a useful model to investigate the function and contribution of beta-defensins to host defense in the lung, kidney, and other tissues."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.org/dc/terms/identifier | "doi:10.1128/iai.67.9.4827-4833.1999"xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.org/dc/terms/identifier | "doi:10.1128/iai.67.9.4827-4833.1999"xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Wang G."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Wang G."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Mills J.N."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Mills J.N."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "McCray P.B. Jr."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "McCray P.B. Jr."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Tack B.F."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Tack B.F."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Nishimura D."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Nishimura D."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Bevins C.L."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Bevins C.L."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Jia H.P."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Jia H.P."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Barahmand-Pour F."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Barahmand-Pour F."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Mallampali R.K."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Mallampali R.K."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Wiles K."xsd:string |
| http://purl.uniprot.org/citations/10456937 | http://purl.uniprot.org/core/author | "Wiles K."xsd:string |