| http://purl.uniprot.org/citations/10471292 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10471292 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10471292 | http://www.w3.org/2000/01/rdf-schema#comment | "We have previously identified a Thr- and Cys-rich thermal hysteresis (antifreeze) protein (THP) in the beetle Tenebrio molitor that has 10-100 times the freezing point depression activity of fish antifreeze proteins. Because this 8.4 kDa protein is significantly different in its properties from THP preparations previously reported from this insect, a thorough search was undertaken for other antifreeze types. Many active proteins were observed, but all appeared to be isoforms of the THP that differed in their number of 12-amino acid repeats (consensus sequence CTxSxxCxxAxT), amino acid substitutions, and N-linked glycosylation. Mass spectral analysis has matched most of these isoforms with cDNA sequences of 17 different clones from a larval fat body library that encode eight different mature THPs containing 84, 96, or 120 amino acids. Genomic Southern blots suggest there may be 30-50 tightly linked copies of the gene, which is a signature consistently seen with unrelated fish antifreeze protein genes, and one that has been associated with the need to rapidly increase gene product in response to climate change. A three-dimensional model is proposed for the fully disulfide-bonded structure of T. molitor THP, which can accommodate addition or deletion of 12-amino acid repeats. The structure is a beta-helix that places most of the Thr in a regular array on one side of the protein to form a putative ice-binding surface."xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi990613s"xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi990613s"xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/author | "Thibault P."xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/author | "Thibault P."xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/author | "Liou Y.C."xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/author | "Liou Y.C."xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/author | "Davies P.L."xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/author | "Davies P.L."xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/author | "Graham L.A."xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/author | "Graham L.A."xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/author | "Walker V.K."xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/author | "Walker V.K."xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/pages | "11415-11424"xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/pages | "11415-11424"xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/title | "A complex family of highly heterogeneous and internally repetitive hyperactive antifreeze proteins from the beetle Tenebrio molitor."xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/title | "A complex family of highly heterogeneous and internally repetitive hyperactive antifreeze proteins from the beetle Tenebrio molitor."xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/volume | "38"xsd:string |
| http://purl.uniprot.org/citations/10471292 | http://purl.uniprot.org/core/volume | "38"xsd:string |