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http://purl.uniprot.org/citations/10485295http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10485295http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10485295http://www.w3.org/2000/01/rdf-schema#comment"The mutant T482 of Sinorhizobium meliloti CXM1-105, which carries a Tn5 insertion on megaplasmid 1, exhibits an enhanced symbiotic efficiency phenotype. Three genes, eglC, cya3 and syrB2, were identified in the eff-482 region tagged by the Tn5 insertion in T482. The eglC gene encodes an endoglycanase which contributes to the depolymerization of the exo-polysaccharide succinoglycan. The N-terminal region of the predicted cya3 gene product was similar to eukaryotic-type adenylate cyclases from Brevibacterium liquefaciens and Streptomyces coelicolor. Four contiguous tetratricopeptide repeats which are known to mediate protein-protein interactions were identified in the C-terminal portion of Cya3. Complementation analysis demonstrated that cya3 indeed encodes a functional adenylate cyclase. A central helix-turn-helix DNA-binding motif and a putative C-terminal coiled-coil structure implicated in protein oligomerization were found in SyrB2. Extra copies of the syrB2 gene negatively affect transcription of both syrB2 itself and cya3. The Tn5 insertion in T482 was localized between the divergently transcribed genes eglC and syrB2. It eliminated eglC function and slightly stimulated transcription of both syrB2 and cya3, which lies downstream of syrB2. Mutants carrying insertions of the lacZ-Gm interposon in the genes eglC, syrB2 and cya3 exhibit the same phenotype as mutant T482, indicating that these three genes influence symbiotic efficiency."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.org/dc/terms/identifier"doi:10.1007/s004380051052"xsd:string
http://purl.uniprot.org/citations/10485295http://purl.org/dc/terms/identifier"doi:10.1007/s004380051052"xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/author"Becker A."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/author"Becker A."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/author"Keller M."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/author"Keller M."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/author"Puehler A."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/author"Puehler A."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/author"Sharypova L.A."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/author"Sharypova L.A."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/author"Simarov B.V."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/author"Simarov B.V."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/author"Yurgel S.N."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/author"Yurgel S.N."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/name"Mol. Gen. Genet."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/name"Mol. Gen. Genet."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/pages"1032-1044"xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/pages"1032-1044"xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/title"The eff-482 locus of Sinorhizobium meliloti CXM1-105 that influences symbiotic effectiveness consists of three genes encoding an endoglycanase, a transcriptional regulator and an adenylate cyclase."xsd:string
http://purl.uniprot.org/citations/10485295http://purl.uniprot.org/core/title"The eff-482 locus of Sinorhizobium meliloti CXM1-105 that influences symbiotic effectiveness consists of three genes encoding an endoglycanase, a transcriptional regulator and an adenylate cyclase."xsd:string