| http://purl.uniprot.org/citations/10485713 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10485713 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10485713 | http://www.w3.org/2000/01/rdf-schema#comment | "Gene activation is a highly regulated process that requires the coordinated action of proteins to relieve chromatin repression and to promote transcriptional activation. Nuclear histone acetyltransferase (HAT) enzymes provide a mechanistic link between chromatin destabilization and gene activation by acetylating the epsilon-amino group of specific lysine residues within the aminoterminal tails of core histones to facilitate access to DNA by transcriptional activators. Here we report the high-resolution crystal structure of the HAT domain of Tetrahymena GCN5 (tGCN5) bound with both its physiologically relevant ligands, coenzyme A (CoA) and a histone H3 peptide, and the structures of nascent tGCN5 and a tGCN5/acetyl-CoA complex. Our structural data reveal histone-binding specificity for a random-coil structure containing a G-K-X-P recognition sequence, and show that CoA is essential for reorienting the enzyme for histone binding. Catalysis appears to involve water-mediated proton extraction from the substrate lysine by a glutamic acid general base and a backbone amide that stabilizes the transition-state reaction intermediate. Comparison with related N-acetyltransferases indicates a conserved structural framework for CoA binding and catalysis, and structural variability in regions associated with substrate-specific binding."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.org/dc/terms/identifier | "doi:10.1038/43487"xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.org/dc/terms/identifier | "doi:10.1038/43487"xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Li X."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Li X."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Marmorstein R."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Marmorstein R."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Zhou J."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Zhou J."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Berger S.L."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Berger S.L."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Allis C.D."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Allis C.D."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Mo Y."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Mo Y."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Trievel R.C."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Trievel R.C."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Rojas J.R."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/author | "Rojas J.R."xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/10485713 | http://purl.uniprot.org/core/name | "Nature"xsd:string |