| http://purl.uniprot.org/citations/10485888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10485888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10485888 | http://www.w3.org/2000/01/rdf-schema#comment | "Genes encoding membrane proteins comprise a substantial proportion of genomes sequenced to date, but ability to perform structural studies on this portion of the proteome is limited. Electrospray ionization-MS (ESI-MS) of an intact protein generates a profile defining the native covalent state of the gene product and its heterogeneity. Here we apply ESI-MS technology with accuracy exceeding 0.01% to a hydrophobic membrane protein with 12-transmembrane alpha-helices, the full-length lactose permease from Escherichia coli. Furthermore, ESI-MS is used to titrate reactive thiols with N-ethylmaleimide. Treatment of the native protein solubilized in detergent micelles reveals only two reactive thiols, and both are protected by a substrate analog."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.96.19.10695"xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.96.19.10695"xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "Lee J.C."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "Lee J.C."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "Faull K.F."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "Faull K.F."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "Whitelegge J.P."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "Whitelegge J.P."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "le Coutre J."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "le Coutre J."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "Kaback H.R."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "Kaback H.R."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "Prive G.G."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "Prive G.G."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "Engel C.K."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/author | "Engel C.K."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/pages | "10695-10698"xsd:string |
| http://purl.uniprot.org/citations/10485888 | http://purl.uniprot.org/core/pages | "10695-10698"xsd:string |