| http://purl.uniprot.org/citations/10499419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10499419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10499419 | http://www.w3.org/2000/01/rdf-schema#comment | "The positions of the disulfide bonds of Selenocosmia huwena lectin-I (SHL-I) from the venom of the Chinese bird spider S. huwena have been determined. The existence of three disulfide bonds in the native SHL-I was proved by matrix-assisted laser desorption ionization time-of-flight mass spectroscopic analysis. To map the disulfide bonds, native SHL-I was proteolytically digested. The resulting peptides were separated by reverse phase high-performance liquid chromatography. Matrix-assisted laser desorption ionization time-of-flight mass spectroscopic analysis indicated the presence of one disulfide bond Cys7-Cys19. The partially reduced peptides by using Tris-(2-carboxyethyl)-phosphine at pH 3.0 were purified by reverse phase high-performance liquid chromatography. Four M Guanidine-HCl was found to increase the yields of partially reduced peptides prominently. The free thiols were carboxamidomethlate by iodoacetamide. The specific location of another disulfide bond Cys2-Cys14 was proved by comparing N-terminal sequencing analysis of the partially reduced and alkylated SHL-I with that of the intact peptide. Finally, the three disulfide linkage of SHL-I could be assigned as Cys2-Cys14, Cys7-Cys19, Cys13-Cys26."xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0196-9781(99)00096-0"xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0196-9781(99)00096-0"xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/author | "Li F."xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/author | "Li F."xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/author | "Liang S.-P."xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/author | "Liang S.-P."xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/name | "Peptides"xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/name | "Peptides"xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/pages | "1027-1034"xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/pages | "1027-1034"xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/title | "Assignment of the three disulfide bonds of Selenocosmia huwena lectin-I from the venom of spider Selenocosmia huwena."xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/title | "Assignment of the three disulfide bonds of Selenocosmia huwena lectin-I from the venom of spider Selenocosmia huwena."xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/10499419 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10499419 |
| http://purl.uniprot.org/citations/10499419 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10499419 |
| http://purl.uniprot.org/citations/10499419 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10499419 |
| http://purl.uniprot.org/citations/10499419 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10499419 |
| http://purl.uniprot.org/uniprot/Q86C51 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10499419 |
| http://purl.uniprot.org/uniprot/Q86C51#attribution-813F412D948AC27C955C8688271A8B18 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/10499419 |