| http://purl.uniprot.org/citations/10500095 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10500095 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10500095 | http://www.w3.org/2000/01/rdf-schema#comment | "Cyclin E is an unstable protein that is degraded in a ubiquitin- and proteasome-dependent pathway. Two factors stimulate cyclin E ubiquitination in vivo: when it is free of its CDK partner, and when it is phosphorylated on threonine 380. We pursued the first of these pathways by using a two-hybrid screen to identify proteins that could bind only to free cyclin E. This resulted in the isolation of human Cul-3, a member of the cullin family of E3 ubiquitin-protein ligases. We found that Cul-3 was bound to cyclin E but not to cyclin E-Cdk2 complexes in mammalian cells, and that overexpression of Cul-3 increased ubiquitination of cyclin E but not other cyclins. Conversely, deletion of the Cul-3 gene in mice caused increased accumulation of cyclin E protein, and had cell-type-specific effects on S-phase regulation. In the extraembryonic ectoderm, in which cells undergo a standard mitotic cycle, there was a greatly increased number of cells in S phase. In the trophectoderm, in which cells go through endocycles, there was a block to entry into S phase. The SCF pathway, which targets cyclins for ubiquitination on the basis of their phosphorylation state, and the Cul-3 pathway, which selects cyclin E for ubiquitination on the basis of its assembly into CDK complexes, may be complementary ways to control cyclin abundance."xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.13.18.2375"xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.13.18.2375"xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/author | "Roberts J.M."xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/author | "Roberts J.M."xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/author | "Singer J.D."xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/author | "Singer J.D."xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/author | "Clurman B."xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/author | "Clurman B."xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/author | "Gurian-West M."xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/author | "Gurian-West M."xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/pages | "2375-2387"xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/pages | "2375-2387"xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/title | "Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells."xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/title | "Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells."xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/volume | "13"xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://purl.uniprot.org/core/volume | "13"xsd:string |
| http://purl.uniprot.org/citations/10500095 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10500095 |
| http://purl.uniprot.org/citations/10500095 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10500095 |