| http://purl.uniprot.org/citations/10506153 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10506153 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10506153 | http://www.w3.org/2000/01/rdf-schema#comment | "Kap104p is a Saccharomyces cerevisiae nuclear import receptor for two essential mRNA-binding proteins, Nab2p and Nab4p/Hrp1p. We demonstrate direct binding of Kap104p to each of these substrates. We have defined the nuclear localization signals in both Nab2p and Nab4p/Hrp1p by Kap104p binding in vitro and KAP104-dependent nuclear import in vivo. The nuclear localization signals map to similar arginine/glycine-rich RNA-binding domains in both proteins and are thus termed rg-nuclear localization signals to distinguish them from classical nuclear localization signals. We also demonstrate that Kap104p, like other known beta-karyopherins (or importins), interacts directly with the small GTPase Ran/Gsp1. However, unlike other known import factors, Ran binding is not sufficient to mediate release of substrates from Kap104p; efficient Ran-GTP-mediated substrate release requires RNA. Also, addition of Kap104p to Nab2p and Nab4p/Hrp1p prebound to single-stranded DNA-cellulose stimulated release of both proteins from the resin. We suggest a simple cycle in which Nab2p and Nab4p/Hrp1p, upon import, are released in the nucleus at sites of transcription by the concerted action of Ran-GTP and binding to newly synthesized mRNA. The resulting ribonucleoprotein complexes are exported to the cytoplasm, where Kap104p rebinds to Nab2p and Nab4p/Hrp1p, contributing to their release from mRNA."xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.41.29031"xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.41.29031"xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/author | "Lee D.C."xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/author | "Lee D.C."xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/author | "Aitchison J.D."xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/author | "Aitchison J.D."xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/pages | "29031-29037"xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/pages | "29031-29037"xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/title | "Kap104p-mediated nuclear import. Nuclear localization signals in mRNA-binding proteins and the role of Ran and RNA."xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/title | "Kap104p-mediated nuclear import. Nuclear localization signals in mRNA-binding proteins and the role of Ran and RNA."xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10506153 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10506153 |
| http://purl.uniprot.org/citations/10506153 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10506153 |
| http://purl.uniprot.org/citations/10506153 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10506153 |
| http://purl.uniprot.org/citations/10506153 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10506153 |
| http://purl.uniprot.org/uniprot/P38217 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10506153 |
| http://purl.uniprot.org/uniprot/P38217#attribution-AE8BE3C87D3ED73FDEA101FEFE0A873C | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/10506153 |