| http://purl.uniprot.org/citations/10524224 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10524224 | http://www.w3.org/2000/01/rdf-schema#comment | "Sparse fur with abnormal skin and hair (spf-ash) mice are deficient in ornithine carbamoyltransferase (OCT) activity, but their OCT protein is kinetically normal. We administered ammonium chloride to spf-ash mice, in order to analyze ammonia metabolism and to find a rationale for the therapy of OCT deficiency. Ammonia concentration in the liver of spf-ash mice increased to a level much higher than in the control. Ammonium chloride injection caused an increase in ornithine (Orn) 5 min after injection and an increase in the sum of Orn, citrulline (Cit) and arginine (Arg) for at least 15 min in the liver of control mice, but no increase in Orn, Cit and Arg in the liver of spf-ash mice. Treatment of spf-ash mice with Arg 5-20 min prior to the injection of ammonium chloride kept the hepatic ammonia concentration at a level comparable to that without the load. A significant reciprocal relationship between ammonia and Orn concentrations in the liver of spf-ash mice 5 min after an ammonium chloride load with or without Arg strongly suggests that ammonia disposal is dependent on the supply of Orn. In spf-ash mice loaded with tryptone as a nitrogen source, Arg supplementation showed a dramatic decrease in urinary orotic acid excretion in a dose-dependent manner. Similar effects were observed with Cit and Orn at the same dose, and a long-lasting effect with an ornithine aminotransferase inactivator, 5-(fluoromethyl)ornithine, at a much lower dose. The rate of urea formation in liver perfused with ammonium chloride was lower in spf-ash mice than in controls, but with the addition of Orn to the medium it increased to a similar level in control and spf-ash mice. These results indicate that OCT is not saturated with Orn in vivo under physiological conditions and that the administration or enrichment of the urea cycle intermediate amino acids enhances the OCT reaction so that the ammonia metabolism of OCT-deficient spf-ash mice is at least partially normalized."xsd:string |
| http://purl.uniprot.org/citations/10524224 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0925-4439(99)00048-4"xsd:string |
| http://purl.uniprot.org/citations/10524224 | http://purl.uniprot.org/core/author | "Nakajima T."xsd:string |
| http://purl.uniprot.org/citations/10524224 | http://purl.uniprot.org/core/author | "Kobayashi K."xsd:string |
| http://purl.uniprot.org/citations/10524224 | http://purl.uniprot.org/core/author | "Li M.X."xsd:string |
| http://purl.uniprot.org/citations/10524224 | http://purl.uniprot.org/core/author | "Fukushige T."xsd:string |
| http://purl.uniprot.org/citations/10524224 | http://purl.uniprot.org/core/author | "Saheki T."xsd:string |
| http://purl.uniprot.org/citations/10524224 | http://purl.uniprot.org/core/author | "Seiler N."xsd:string |
| http://purl.uniprot.org/citations/10524224 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10524224 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/10524224 | http://purl.uniprot.org/core/pages | "1-11"xsd:string |
| http://purl.uniprot.org/citations/10524224 | http://purl.uniprot.org/core/title | "Aberrations of ammonia metabolism in ornithine carbamoyltransferase-deficient spf-ash mice and their prevention by treatment with urea cycle intermediate amino acids and an ornithine aminotransferase inactivator."xsd:string |
| http://purl.uniprot.org/citations/10524224 | http://purl.uniprot.org/core/volume | "1455"xsd:string |
| http://purl.uniprot.org/citations/10524224 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10524224 |
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