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http://purl.uniprot.org/citations/10545099http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10545099http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10545099http://www.w3.org/2000/01/rdf-schema#comment"mukF, mukE and mukB genes are essential for the process of chromosome partitioning in Escherichia coli. We have studied protein-protein interactions among MukB, MukE and MukF proteins by co-immunoprecipitation and sucrose gradient sedimentation experiments, using mukFEB null cells harboring plasmids carrying the wild-type or mutant-type mukFEB operon. MukB forms a complex with MukF and MukE. Analysis of mutant MukB proteins suggested that MukF and MukE bind the C-terminal globular domain of MukB. MukF is indispensable for an interaction between MukB and MukE; however, MukF itself is able to associate with MukB even in the absence of MukE. We have also found that MukF has a Ca(2+)-binding activity. Although purified MukF was able to make a complex either with MukE or MukB, a complex consisting of the three Muk proteins was barely detected in vitro. However, increasing the Ca(2+) or Mg(2+) concentration in the reaction partially restored complex formation. This suggests that Ca(2+) or Mg(2+) may be required for the formation of a complex consisting of the three Muk proteins, and thus may participate in a particular step during chromosome partitioning."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.org/dc/terms/identifier"doi:10.1093/emboj/18.21.5873"xsd:string
http://purl.uniprot.org/citations/10545099http://purl.org/dc/terms/identifier"doi:10.1093/emboj/18.21.5873"xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Ichimura T."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Ichimura T."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Yamanaka K."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Yamanaka K."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Hiraga S."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Hiraga S."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Niki H."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Niki H."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Yamazoe M."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Yamazoe M."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Onogi T."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Onogi T."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Sunako Y."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/author"Sunako Y."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/name"EMBO J."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/name"EMBO J."xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/pages"5873-5884"xsd:string
http://purl.uniprot.org/citations/10545099http://purl.uniprot.org/core/pages"5873-5884"xsd:string