| http://purl.uniprot.org/citations/10551881 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10551881 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10551881 | http://www.w3.org/2000/01/rdf-schema#comment | "The genome of Escherichia coli is composed of a single molecule of circular DNA with the length of about 47,000 kilobase pairs, which is associated with about 10 major DNA-binding proteins, altogether forming the nucleoid. We expressed and purified 12 species of the DNA-binding protein, i.e. CbpA (curved DNA-binding protein A), CbpB or Rob (curved DNA-binding protein B or right arm of the replication origin binding protein), DnaA (DNA-binding protein A), Dps (DNA-binding protein from starved cells), Fis (factor for inversion stimulation), Hfq (host factor for phage Q(beta)), H-NS (histone-like nucleoid structuring protein), HU (heat-unstable nucleoid protein), IciA (inhibitor of chromosome initiation A), IHF (integration host factor), Lrp (leucine-responsive regulatory protein), and StpA (suppressor of td(-) phenotype A). The sequence specificity of DNA binding was determined for all the purified nucleoid proteins using gel-mobility shift assays. Five proteins (CbpB, DnaA, Fis, IHF, and Lrp) were found to bind to specific DNA sequences, while the remaining seven proteins (CbpA, Dps, Hfq, H-NS, HU, IciA, and StpA) showed apparently sequence-nonspecific DNA binding activities. Four proteins, CbpA, Hfq, H-NS, and IciA, showed the binding preference for the curved DNA. From the apparent dissociation constant (K(d)) determined using the sequence-specific or nonspecific DNA probes, the order of DNA binding affinity were determined to be: HU > IHF > Lrp > CbpB(Rob) > Fis > H-NS > StpA > CbpA > IciA > Hfq/Dps, ranging from 25 nM (HU binding to the non-curved DNA) to 250 nM (Hfq binding to the non-curved DNA), under the assay conditions employed."xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.46.33105"xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.46.33105"xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/author | "Ishihama A."xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/author | "Ishihama A."xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/author | "Azam T.A."xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/author | "Azam T.A."xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/pages | "33105-33113"xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/pages | "33105-33113"xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/title | "Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity."xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/title | "Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity."xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10551881 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10551881 |
| http://purl.uniprot.org/citations/10551881 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10551881 |
| http://purl.uniprot.org/citations/10551881 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10551881 |
| http://purl.uniprot.org/citations/10551881 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10551881 |
| http://purl.uniprot.org/uniprot/P36659 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10551881 |
| http://purl.uniprot.org/uniprot/P0A8S1 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10551881 |