| http://purl.uniprot.org/citations/10558878 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10558878 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10558878 | http://www.w3.org/2000/01/rdf-schema#comment | "DNase II is a well-known deoxyribonuclease (DNase) that catalyzes the hydrolysis of DNA into oligonucleotides under acidic conditions. We have identified a novel DNase that shows homology to DNase II, named DLAD, from a search of an expressed sequence tag data base. The full-length cDNA for rat DLAD cloned by polymerase chain reaction encodes a 356-amino acid polypeptide containing a putative N-terminal signal peptide and 5 potential N-glycosylation sites; there is a predicted catalytic domain resemblance to rat DNase II. The predicted DLAD translation product shares 32.9% identity with DNase II. Interestingly, expression of the DRAD mRNA is highly restricted to the liver. A Myc-His tagged recombinant DLAD recovered mainly from the cytoplasm of transfected HeLa S3 cells has a divalent cation-independent DNase activity. The DLAD activity prefers acidic conditions to neutral. The recombinant protein expressed in HeLa S3 cells inhibits the expression of GFP- and lac Z-expression vectors, suggesting that DLAD may play a role in elimination of exogenous DNA. Identification of the full-length cDNA for DLAD would lead to an understanding of the physiology of this DNase II-like molecule."xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.1999.1699"xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.1999.1699"xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/author | "Tanuma S."xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/author | "Tanuma S."xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/author | "Shiokawa D."xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/author | "Shiokawa D."xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/pages | "395-399"xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/pages | "395-399"xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/title | "Cloning of a cDNA encoding a rat DNase II-like acid DNase."xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/title | "Cloning of a cDNA encoding a rat DNase II-like acid DNase."xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/volume | "265"xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://purl.uniprot.org/core/volume | "265"xsd:string |
| http://purl.uniprot.org/citations/10558878 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10558878 |
| http://purl.uniprot.org/citations/10558878 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10558878 |
| http://purl.uniprot.org/citations/10558878 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10558878 |
| http://purl.uniprot.org/citations/10558878 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10558878 |
| http://purl.uniprot.org/uniprot/Q9QZK8 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10558878 |
| http://purl.uniprot.org/uniprot/Q9QZK9 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10558878 |