| http://purl.uniprot.org/citations/10586881 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10586881 | http://www.w3.org/2000/01/rdf-schema#comment | "Economical methods by which gene function may be analysed on a genomic scale are relatively scarce. To fill this need, we have developed a transposon-tagging strategy for the genome-wide analysis of disruption phenotypes, gene expression and protein localization, and have applied this method to the large-scale analysis of gene function in the budding yeast Saccharomyces cerevisiae. Here we present the largest collection of defined yeast mutants ever generated within a single genetic background--a collection of over 11,000 strains, each carrying a transposon inserted within a region of the genome expressed during vegetative growth and/or sporulation. These insertions affect nearly 2,000 annotated genes, representing about one-third of the 6,200 predicted genes in the yeast genome. We have used this collection to determine disruption phenotypes for nearly 8,000 strains using 20 different growth conditions; the resulting data sets were clustered to identify groups of functionally related genes. We have also identified over 300 previously non-annotated open reading frames and analysed by indirect immunofluorescence over 1,300 transposon-tagged proteins. In total, our study encompasses over 260,000 data points, constituting the largest functional analysis of the yeast genome ever undertaken."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.org/dc/terms/identifier | "doi:10.1038/46558"xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Gerstein M."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Agarwal S."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Iwasaki H."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Kumar A."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Snyder M."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Heidtman M."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Jansen R."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Miller P."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Roeder G.S."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Roemer T."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Umansky L."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Sheehan A."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Cheung K.H."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Ross-Macdonald P."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Hager K."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Nelson F.K."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Coelho P.S."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/author | "Symoniatis D."xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/pages | "413-418"xsd:string |
| http://purl.uniprot.org/citations/10586881 | http://purl.uniprot.org/core/title | "Large-scale analysis of the yeast genome by transposon tagging and gene disruption."xsd:string |