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http://purl.uniprot.org/citations/10589015http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10589015http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10589015http://www.w3.org/2000/01/rdf-schema#comment"

Background

Soybean proteins are constituents of a number of food products and represent a panel of potential allergens. Thus far, little is known about the molecular characteristics of soybean allergens.

Objective

The aim of this study was to identify the soybean profilin by PCR-based complementary (c)DNA cloning and to elucidate its allergenic characteristics.

Methods

Highly degenerate profilin-specific primers were used to identify, by means of PCR, 2 soybean profilin isoforms (GmPRO1 and GmPRO2) by using soybean cDNA as a target. One isoform (GmPRO1) with a length of 394 bp corresponding to 131 amino acid residues was subcloned and expressed in fusion with the maltose-binding protein. Moreover, 3 overlapping recombinant soybean profilin fragments comprising amino acid residues 1-65, 38-88, and 50-131 were also prepared as maltose-binding protein fusion proteins. IgE-binding reactivity of the recombinant proteins and the cross-reactivity of soybean profilin with birch profilin was studied by immunoblotting, enzyme-linked allergosorbent assays (EASTs), and competitive inhibition experiments by using serum samples from 13 soybean-sensitized subjects.

Results

Results of immunoblot analysis, EAST, and EAST-inhibition experiments indicate the presence of profilin in soybean extract. The recombinant soybean profilin (rGly m 3) was recognized by IgE in 9 (69%) of the 13 sera tested. Only the full-length rGly m 3 was able to bind with IgE antibodies, whereas the 3 soybean profilin fragments did not show significant binding reactivity, indicating that the IgE binding to rGly m 3 depends on the integrity of a conformational structure, which was not present in the overlapping profilin fragments. The rGly m 3 cross-reacted with birch pollen profilin (Bet v 2), and the IgE binding to Bet v 2 could be inhibited by rGly m 3.

Conclusions

rGly m 3 represents a new soybean allergen with well-characterized primary sequence, and its IgE-binding reactivity is mediated by conformational epitopes."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.org/dc/terms/identifier"doi:10.1016/s0091-6749(99)70027-8"xsd:string
http://purl.uniprot.org/citations/10589015http://purl.org/dc/terms/identifier"doi:10.1016/s0091-6749(99)70027-8"xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Chen Z."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Chen Z."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Petersen A."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Petersen A."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Baur X."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Baur X."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Rozynek P."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Rozynek P."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Heimann H."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Heimann H."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Rihs H.-P."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Rihs H.-P."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Rueff F."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/author"Rueff F."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/name"J. Allergy Clin. Immunol."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/name"J. Allergy Clin. Immunol."xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/pages"1293-1301"xsd:string
http://purl.uniprot.org/citations/10589015http://purl.uniprot.org/core/pages"1293-1301"xsd:string
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