| http://purl.uniprot.org/citations/10601301 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10601301 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10601301 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/10601301 | http://www.w3.org/2000/01/rdf-schema#comment | "Arachidonic (20:4(n-6)), eicosapentaenoic (20:5(n-3)), and docosahexaenoic (22:6(n-3)) acids are major components of brain and retina phospholipids, substrates for eicosanoid production, and regulators of nuclear transcription factors. One of the two rate-limiting steps in the production of these polyenoic fatty acids is the desaturation of 20:3(n-6) and 20:4(n-3) by Delta-5 desaturase. This report describes the cloning and expression of the human Delta-5 desaturase, and it compares the structural characteristics and nutritional regulation of the Delta-5 and Delta-6 desaturases. The open reading frame of the human Delta-5 desaturase encodes a 444-amino acid peptide which is identical in size to the Delta-6 desaturase and which shares 61% identity with the human Delta-6 desaturase. The Delta-5 desaturase contains two membrane-spanning domains, three histidine-rich regions, and a cytochrome b(5) domain that all align perfectly with the same domains located in the Delta-6 desaturase. Expression of the open reading frame in Chinese hamster ovary cells instilled the ability to convert 20:3(n-6) to 20:4(n-6). Northern analysis revealed that many human tissues including skeletal muscle, lung, placenta, kidney, and pancreas expressed Delta-5 desaturase mRNA, but Delta-5 desaturase was most abundant in the liver, brain, and heart. However, in all tissues, the abundance of Delta-5 desaturase mRNA was much lower than that observed for the Delta-6 desaturase. When rats were fed a diet containing 10% safflower oil or menhaden fish oil, the level of hepatic mRNA for Delta-5 and Delta-6 desaturase was only 25% of that found in the liver of rats fed a fat-free diet or a diet containing triolein. Finally, a BLAST and Genemap search of the human genome revealed that the Delta-5 and Delta-6 desaturase genes reside in reverse orientation on chromosome 11 and that they are separated by <11,000 base pairs."xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.52.37335"xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.52.37335"xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.52.37335"xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/author | "Nakamura M."xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/author | "Nakamura M."xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/author | "Clarke S.D."xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/author | "Clarke S.D."xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/author | "Cho H.P."xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/author | "Cho H.P."xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/pages | "37335-37339"xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/pages | "37335-37339"xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/title | "Cloning, expression, and fatty acid regulation of the human Delta-5 desaturase."xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/title | "Cloning, expression, and fatty acid regulation of the human Delta-5 desaturase."xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://purl.uniprot.org/core/volume | "274"xsd:string |
| http://purl.uniprot.org/citations/10601301 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10601301 |
| http://purl.uniprot.org/citations/10601301 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10601301 |