| http://purl.uniprot.org/citations/10606664 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10606664 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10606664 | http://www.w3.org/2000/01/rdf-schema#comment | "Snail family proteins are zinc finger transcriptional regulators first identified in Drosophila which play critical roles in cell fate determination. We identified a novel Snail -related gene from murine skeletalmusclecells designated Smuc. Northern blot analysis showed that Smuc was highly expressed in skeletal muscle and thymus. Smuc contains five putative DNA-binding zinc finger domains in its C-terminal half. In electrophoretic mobility shift assays, recombinant zinc finger domains of Smuc specifically bound to CAGGTG and CACCTG E-box motifs (CANNTG). Because basic helix-loop-helix transcription factors (bHLH) bind to the same E-box sequences, we examined whether Smuc competes with the myogenic bHLH factor MyoD for DNA binding. Smuc inhibited the binding of a MyoD-E12 complex to the CACCTG E-box sequence in a dose-dependent manner and suppressed the transcriptional activity of MyoD-E12. When heterologously targeted to the thymidine kinase promoter as fusion proteins with the GAL4 DNA-binding domain, the non-zinc finger domain of Smuc acted as a transcriptional repressor. Furthermore, overexpression of Smuc in myoblasts repressed transactivation of muscle differentiation marker Troponin T. Thus, Smuc might regulate bHLH transcription factors by zinc finger domains competing for E-box binding, and non-zinc finger repressor domains might also confer transcriptional repression to control differentiation processes."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/28.2.626"xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/28.2.626"xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Kataoka H."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Kataoka H."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Mori S."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Mori S."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Nishikawa S."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Nishikawa S."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Ozaki H."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Ozaki H."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Sano H."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Sano H."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Kita T."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Kita T."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Yokota Y."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Yokota Y."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Murayama T."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Murayama T."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Yokode M."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/author | "Yokode M."xsd:string |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10606664 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |